Proteomic Analysis of Hepatic Tissue of Zebrafish (Danio rerio) Experimentally Exposed to Chronic Microcystin-LR

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	Proteomic Analysis of Hepatic Tissue of Zebrafish (Danio rerio) Experimentally Exposed to Chronic Microcystin-LR
	Wang, M. H. ; Chan, L. L. ; Si, M. Z. ; Hong, H. S. ; Wang, D. Z. ; Wang DZ(王大志)
刊名	http://dx.doi.org/10.1093/toxsci/kfp248
	2010-01
关键词	PROTEIN PHOSPHATASE-ACTIVITY CARP CYPRINUS-CARPIO CARASSIUS-AURATUS L ARABIDOPSIS-THALIANA OXIDATIVE STRESS KINASE-C LIVER IDENTIFICATION HEPATOCYTES FISH
英文摘要	Xiamen University; National Natural Science Foundation of China [40806051]; Microcystin-LR (MCLR) is the most toxic and most frequently encountered hepatotoxin in the aquatic environment. This study investigated the protein profiles of zebrafish (Danio rerio) livers chronically exposed to MCLR concentrations (2 or 20 mu g/l) using the proteomic approach as well as cell ultrastructure, protein phosphatase (PP) activity, protein phosphatase 2A (PP2A) abundance, and toxin content analysis of the hepatic tissue. The results showed that, after 30-day exposure, the presence of MCLR strikingly enhanced toxin accumulation and the PP activity in zebrafish livers. However, the PP2A amounts were independent of toxin treatments. MCLR caused a noticeable damage to liver ultrastructure, a widespread swelling in the rough endoplasmatic reticulum and mitochondria was observed in the MCLR-exposed hepatocytes, and a honeycomb-like structure was formed in the treated nucleoli. Comparison of two-dimensional electrophoresis (2-DE) protein profiles of MCLR-exposed and nonexposed zebrafish livers revealed that the abundance of 22 proteins, measured by 2-DE, was remarkably altered in response to toxin exposure. These proteins were involved in cytoskeleton assembly, macromolecule metabolism, oxidative stress, and signal transduction, indicating that MCLR toxicity in fish liver is complex and diverse. Thus, proteomics provides a new insight into MCLR toxicity, that chronic toxicity of MCLR is different from acute toxicity, and we speculate that the reactive oxygen species pathway might be the main toxic pathway instead of the PP one. Moreover, even a low concentration of MCLR in water could significantly interrupt cellular processes, and more care should be taken in determining the criterion for MCLR content in drinking water.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/60579]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Wang, M. H.,Chan, L. L.,Si, M. Z.,et al. Proteomic Analysis of Hepatic Tissue of Zebrafish (Danio rerio) Experimentally Exposed to Chronic Microcystin-LR[J]. http://dx.doi.org/10.1093/toxsci/kfp248,2010.
APA	Wang, M. H.,Chan, L. L.,Si, M. Z.,Hong, H. S.,Wang, D. Z.,&王大志.(2010).Proteomic Analysis of Hepatic Tissue of Zebrafish (Danio rerio) Experimentally Exposed to Chronic Microcystin-LR.http://dx.doi.org/10.1093/toxsci/kfp248.
MLA	Wang, M. H.,et al."Proteomic Analysis of Hepatic Tissue of Zebrafish (Danio rerio) Experimentally Exposed to Chronic Microcystin-LR".http://dx.doi.org/10.1093/toxsci/kfp248 (2010).