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	Effects of UVB on the expression of proliferating cell nuclear antigen (PCNA) in dinoflagellate Prorocentrum donghaiense Lu
	Liu, J. W. ; Jiao, N. Z. ; Cai, H. N. ; Hong, H. S. ; Jiao NZ(焦念志)
	2007
关键词	ULTRAVIOLET-B RADIATION MARINE-PHYTOPLANKTON OZONE DEPLETION ANTARCTIC PHYTOPLANKTON DNA-DAMAGE BACILLARIOPHYCEAE PHOTOSYNTHESIS PROTEINS GENE
英文摘要	PCNA has been considered as a useful marker for the estimation of growth rates of marine phytoplankton at the species level. Since dinoflagellates are noted for having many prokaryotic features in that they are the only eukaryotes to have permanently condensed chromosomes as well as lacking histones and a nucleosome, the sensitivity to UVB radiation and the validity of PCNA as a maker of growth rate in dinoflagellate need to be evaluated. Prorocentrum donghaiense Lu was investigated to valuate the UVB sensitivity in relation to cellular and molecular aspects of PCNA as a growth indicator. The effects of UVB radiation on PCNA were studied using the methods of western blots technology and whole-cell immunoflurescence with one mono-antibody. UVB changed the cell morphology, halted the growth and increased the cell size, even caused cell death to a certain extent after treatment with UVB radiation in P. donghaiense. Compared with the control, treating the algal cultures in exponential phases with UVB radiation for 24 h caused chromatin release and increases in protein levels of PCNA.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/60503]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Liu, J. W.,Jiao, N. Z.,Cai, H. N.,et al. Effects of UVB on the expression of proliferating cell nuclear antigen (PCNA) in dinoflagellate Prorocentrum donghaiense Lu[J],2007.
APA	Liu, J. W.,Jiao, N. Z.,Cai, H. N.,Hong, H. S.,&焦念志.(2007).Effects of UVB on the expression of proliferating cell nuclear antigen (PCNA) in dinoflagellate Prorocentrum donghaiense Lu..
MLA	Liu, J. W.,et al."Effects of UVB on the expression of proliferating cell nuclear antigen (PCNA) in dinoflagellate Prorocentrum donghaiense Lu".(2007).

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