Characterization and bacterial-binding activity of a novel C-type lectin from the red-spotted grouper, Epinephelus akaara

CORC > 厦门大学 > 海洋环境－已发表论文

	Characterization and bacterial-binding activity of a novel C-type lectin from the red-spotted grouper, Epinephelus akaara
	Zhang, Z. W. ; Shi, X. F. ; Qu, M. ; Ding, S. X. ; Ding SX(丁少雄)
刊名	http://dx.doi.org/10.4238/2012.May.11.1
	2012
关键词	PATTERN-RECOGNITION MOLECULES INNATE IMMUNE-RESPONSE COMPLEMENT-SYSTEM ADAPTIVE IMMUNITY RAINBOW-TROUT 2 HOMOLOGS FISH PATHWAY IDENTIFICATION EVOLUTION
英文摘要	Nature Science Foundation of Fujian Province of China [2010J01226]; project of Seed industry innovation and industrialization of Fujian Province of China; Because of their specific binding to carbohydrates, lectins play a crucial role in pathogen recognition and clearance in vertebrate animals. Previously, only two types of collectins had been isolated from bony fish: mannan-binding lectin (MBL) and galactose-binding lectin (GalBL). We sequenced a novel collectin (designated EALec1) from the red-spotted grouper, Epinephelus akaara. The gene structure of EALec1 and the alignment of the carbohydrate recognition domain of the three collectins demonstrated that EALec1 is a new type of collectin derived from MBL. We examined the expression pattern of the EALec1 transcripts in 12 tissues of the red-spotted grouper. The EALec1 gene was found to have multiple copies; their transcripts were detected in all 12 tissues. EALec1 was also recombined and expressed in Escherichia coli to investigate its immune functions and carbohydrate-binding characterization. We concluded that EALec1 belongs to the mannan-binding lectin group, despite its different Ca2+-dependent sites in the carbohydrate recognition domain, and that it is involved in the recognition and clearance of invaders in the red-spotted grouper.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/60068]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Zhang, Z. W.,Shi, X. F.,Qu, M.,et al. Characterization and bacterial-binding activity of a novel C-type lectin from the red-spotted grouper, Epinephelus akaara[J]. http://dx.doi.org/10.4238/2012.May.11.1,2012.
APA	Zhang, Z. W.,Shi, X. F.,Qu, M.,Ding, S. X.,&丁少雄.(2012).Characterization and bacterial-binding activity of a novel C-type lectin from the red-spotted grouper, Epinephelus akaara.http://dx.doi.org/10.4238/2012.May.11.1.
MLA	Zhang, Z. W.,et al."Characterization and bacterial-binding activity of a novel C-type lectin from the red-spotted grouper, Epinephelus akaara".http://dx.doi.org/10.4238/2012.May.11.1 (2012).