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	Quantitative proteomic analysis of okadaic acid treated mouse small intestines reveals differentially expressed proteins involved in diarrhetic shellfish poisoning
	Wang, Juan ; Wang, Yu-Yu ; Lin, Lin ; Gao, Yue ; Hong, Hua-Sheng ; Hong HS(洪华生) ; Wang, Da-Zhi ; Wang DZ(王大志)
	2012-04-03
关键词	Okadaic acid Diarrhetic shellfish poisoning Protein phosphatase Ultrastructure Proteomics Two-dimensional difference gel electrophoresis
英文摘要	Okadaic acid (OA) is a principal diarrhetic shellfish poisoning toxin produced by marine dinoflagellates. This study compared protein profiles of mice small intestines at four time points (0, 3, 6 and 24 h) after a single oral administration of 750 mu g/kg OA, and identified the differentially expressed proteins using 2-D DIGE and MALDI-TOF-TOF mass spectrometry. The results showed that the toxin content of the intestines reached its peak 3 h after oral administration and then decreased rapidly. OA remarkably inhibited the intestinal PP activity but it recovered to the normal levels within 6 to 24 h. Electron microscope revealed the collapse of the villous architecture and the intestinal microvilli fell off at 3 h, but were repaired within 24 h. Notable damage to the intestinal ultrastructure was observed after oral administration. Comparison of the small intestine protein profiles at four time points revealed that 58 proteins were remarkably altered in abundance, and these proteins were involved in macromolecular metabolism, cytoskeleton reorganization, signal transduction, molecular chaperoning and oxidative stress, suggesting that OA toxicity in mouse intestines was complex and diverse, and that multiple proteins other than PP were involved in the diarrhetic process. Villin 1 and hnRNP F might be the key triggers inducing diarrhea in the mouse small intestines. (C) 2012 Elsevier B.V. All rights reserved.; State Key Laboratory of Marine Environmental Science (Xiamen University); National Natural Science Foundation of China [41106092]; Program for New Century Excellent Talents in Universities of China
语种	英语
出版者	ELSEVIER SCIENCE BV
内容类型	期刊论文
源URL	[http://dx.doi.org/10.1016/j.jprot.2012.01.010]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Wang, Juan,Wang, Yu-Yu,Lin, Lin,et al. Quantitative proteomic analysis of okadaic acid treated mouse small intestines reveals differentially expressed proteins involved in diarrhetic shellfish poisoning[J],2012.
APA	Wang, Juan.,Wang, Yu-Yu.,Lin, Lin.,Gao, Yue.,Hong, Hua-Sheng.,...&王大志.(2012).Quantitative proteomic analysis of okadaic acid treated mouse small intestines reveals differentially expressed proteins involved in diarrhetic shellfish poisoning..
MLA	Wang, Juan,et al."Quantitative proteomic analysis of okadaic acid treated mouse small intestines reveals differentially expressed proteins involved in diarrhetic shellfish poisoning".(2012).

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