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	Development and evaluation of specific 16S rDNA primers for marine Cytophaga-Flavobacteria cluster
	Chen, Xihan ; Zeng, Yonghui ; Jiao, Nianzhi ; Jiao NZ(焦念志)
	2006-09-21
关键词	16S rDNA clone library Cytophaga-Flavobacteria diversity marine environments primer design
英文摘要	Through multiple alignment analysis of 16S rDNA sequences from all known genera of Cytophaga-Flavobacteria (CF) cluster, a new primer pair specifically targeting this cluster was developed, greatly facilitating their diversity and function's exploration in marine ecosystems. Compared with previously reported primers, the new primer pair could theoretically retrieve broader CF diversity without decreasing specificity. The effectiveness for field samples was further evaluated by testing the community DNA samples from various marine environments using the optimal polymerase chain reaction (PCR) conditions established in this work. The results showed its robustness and high specificity for amplifying CF cluster's 16S rDNA fragments from complex marine environments.
语种	英语
出版者	BLACKWELL PUBLISHING
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1111/j.1471-8286.2006.01514.x]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Chen, Xihan,Zeng, Yonghui,Jiao, Nianzhi,et al. Development and evaluation of specific 16S rDNA primers for marine Cytophaga-Flavobacteria cluster[J],2006.
APA	Chen, Xihan,Zeng, Yonghui,Jiao, Nianzhi,&焦念志.(2006).Development and evaluation of specific 16S rDNA primers for marine Cytophaga-Flavobacteria cluster..
MLA	Chen, Xihan,et al."Development and evaluation of specific 16S rDNA primers for marine Cytophaga-Flavobacteria cluster".(2006).

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