Influence of LDAO on the Conformation and Release of Bacteriochlorophyll of Peripheral Light-Harvesting Complex (LH2) from Rhodobacter Azotoformans | |
Zhao, Gen-gui ( Huaqiao Univ, Dept Bioengn & Biotechnol, Xiamen 361021, Peoples R China ) ; Dong, Yan-min ( Shanxi Univ, Key Lab Chem Biol & Mol Engn, Minist Educ, Sch Life Sci & Technol, Taiyuan 030006, Peoples R China) ; Yang, Su-ping(Huaqiao Univ, Dept Bioengn & Biotechnol, Xiamen 361021, Peoples R China) ; Jiao, Nian-zhi ; Jiao NZ(焦念志) ; Qu, Yin-bo(Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China) | |
2010-10 | |
关键词 | Peripheral light-harvesting complex Bacteriochlorophyll LDAO |
英文摘要 | The aim of this study is to reveal the interaction relationships between lauryl dimethylamine N-oxide (LDAO) and peripheral light-harvesting complex (LH2) as well as the influence of LDAO on structure and function of LH2. In the present work, the effects of LDAO on the conformation and release processes of bacteriochlorophyll (BChl) of LH2 when incubated under different temperature and pH in the presence and absence of LDAO were investigated by spectroscopy. The results indicated that (1) the presence of LDAO resulted in alterations in the conformation, a-helix content, and spectra of Tyr and B850 band of LH2 at room temperature and pH 8. 0. Moreover, energy transfer efficiency of LH2 was enhanced markedly in the presence of LDAO. (2) At 60 degrees C, both the 13800 and 13850 band of LH2 were released and transited into free BChl at pH 8. 0. However, the release rates of bacteriochlorophylls of B800 and 13850 band from LH2 were slowed clown and the release processes were changed when incubated in the presence of LDAO. Hence, the stability of LH2 was improved in the presence of LDAO. (3) The accelerated release processes of bacteriochlorophylls of B800 and 13850 band of LH2 were induced to transform into bacteriopheophytin (BPhe) and free BChl by LDAO in strong acid and strong alkalic solution at room temperature. However, the kinetic patterns of the B800 and B850 band were remarkably different. The release and self-assemble processes of B850 in LH2 were observed in strong acid solution without LDAO. Therefore, the different release behaviors of 13800 and 13850 of LH2 are induced by LDAO under different extreme environmental conditions. |
语种 | 英语 |
出版者 | OFFICE SPECTROSCOPY & SPECTRAL ANALYSIS |
内容类型 | 期刊论文 |
源URL | [http://dx.doi.org/doi:10.3964/j.issn.1000-0593(2010)10-2807-05] ![]() |
专题 | 海洋环境-已发表论文 |
推荐引用方式 GB/T 7714 | Zhao, Gen-gui ,Dong, Yan-min ,Yang, Su-ping,et al. Influence of LDAO on the Conformation and Release of Bacteriochlorophyll of Peripheral Light-Harvesting Complex (LH2) from Rhodobacter Azotoformans[J],2010. |
APA | Zhao, Gen-gui ,Dong, Yan-min ,Yang, Su-ping,Jiao, Nian-zhi,焦念志,&Qu, Yin-bo.(2010).Influence of LDAO on the Conformation and Release of Bacteriochlorophyll of Peripheral Light-Harvesting Complex (LH2) from Rhodobacter Azotoformans.. |
MLA | Zhao, Gen-gui ,et al."Influence of LDAO on the Conformation and Release of Bacteriochlorophyll of Peripheral Light-Harvesting Complex (LH2) from Rhodobacter Azotoformans".(2010). |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论