QQ客服 官方微博 反馈留言

	电堆积柱上富集-高效毛细管电泳法测定药品中盐酸麻黄碱和盐酸伪麻黄碱的含量; HPCE Determination of Ephedrine Hydrochloride and Pseudo-Ephedrine Hydrochloride in Drugs with Enrichment by Electro-stacking
	涂逢樟 ; 姚辉梅 ; 钟春龙 ; 卢艺平 ; 林竹光
	2011
关键词	高效毛细管电泳法 电堆积 盐酸麻黄碱 盐酸伪麻黄碱 HPCE Electro-stacking Ephedrine hydrochloride Pseudo-ephedrine hydrochloride
英文摘要	应用电堆积柱上富集-高效毛细管电泳法测定了6种药品中盐酸麻黄碱和盐酸伪麻黄碱含量。试验选择了以下分析条件:1检测波长205 nM;2内标物为间苯二酚;3运行液为PH9.2的40 MMOl·l-1硼砂缓冲溶液;4分离电压20 kV;5进样时间10 S;6分离温度25℃。麻黄碱及伪麻黄碱质量浓度在1--400 Mg·l-1之间与相应的相对峰面积值(即被测物与内标物的峰面积之比)呈线性关系。方法的检出限(3S/n)为0.35 Mg·l-1(麻黄碱)和0.29 Mg·l-1(伪麻黄碱)。以2种药品作基体加入标准溶液做回收试验,测得平均回收率依次为101.1%及103.6%。; HPCE with electro-stacking enrichment was applied to the determination of ephedrine hydrochloride and pseudo-ephedrine hydrochloride in 6 drugs.Analytical conditions were studied and following optimized parameters were given: ① wavelength for UV-detection: 205 nm;② internal standard: resorcinol;③ electrophoretic medium: 40 mmol·L-1 borax solution(pH 9.2);④ separation voltage: 20 kV;⑤ time of sample introduction: 10 s;⑥ temperature of capillary: 25 ℃.Linear relationships between values of relative peak area(i.e.,ratio of peak area of the analyte to peak area of internal standard) and concentration of ephedrine and pseudo-ephedrine were kept in the same range of 1 to 400 mg·L-1,with detection limits(3S/N) of 0.35 mg·L-1 and 0.29 mg·L-1 respectively.Recovery was tested by standard addition method using 2 drug samples as matrixes,giving values of average recovery of 101.1%(for ephedrine) and 103.6%(for pseudo-ephedrine).; 龙岩学院第二批教育教学改革立项项目(岩学院教[2009]40号)资助
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/98375]
专题	材料学院－已发表论文
推荐引用方式 GB/T 7714	涂逢樟,姚辉梅,钟春龙,等. 电堆积柱上富集-高效毛细管电泳法测定药品中盐酸麻黄碱和盐酸伪麻黄碱的含量, HPCE Determination of Ephedrine Hydrochloride and Pseudo-Ephedrine Hydrochloride in Drugs with Enrichment by Electro-stacking[J],2011.
APA	涂逢樟,姚辉梅,钟春龙,卢艺平,&林竹光.(2011).电堆积柱上富集-高效毛细管电泳法测定药品中盐酸麻黄碱和盐酸伪麻黄碱的含量..
MLA	涂逢樟,et al."电堆积柱上富集-高效毛细管电泳法测定药品中盐酸麻黄碱和盐酸伪麻黄碱的含量".(2011).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。