Multi-walled carbon nanotubes induce apoptosis in RAW 2647 cell-derived osteoclasts through mitochondria-mediated death pathway

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	Multi-walled carbon nanotubes induce apoptosis in RAW 2647 cell-derived osteoclasts through mitochondria-mediated death pathway
	Ye, Shefang ; Jiang, Yuanq ; Zhang, Honggang ; Wang, Yifang ; Wu, Yihui ; Hou, Zhenqing ; Zhang, Qiqi ; Ye SF(叶社房) ; Hou ZQ(侯振清) ; Zhang QQ(张其清)
刊名	http://dx.doi.org/10.1166/jnn.2012.5677
	2012
关键词	Bone Cell culture Chemical activation Mitochondria Multiwalled carbon nanotubes (MWCN) Tissue Tissue engineering
英文摘要	Carbon nanotubes (CNTs) have attracted great interest with respect to biomaterials, particularly for use as an implant material in bone-tissue engineering. Accordingly, the bone-tissue compatibility of CNTs and their influence on new bone formation are important issues. In the present study, we examined the effects of multi-wall carbon nanotubes (MWCNTs) on the receptor activator of nuclear factor 魏B ligand (RANKL)-supported osteoclastogenesis using a murine monocytic cell line RAW 264.7. MWCNTs significantly suppressed the differentiation of RAW 264.7 cells into osteoclasts. Treatment with MWCNTs induced apoptosis in osteoclasts as characterized by nuclear condensation, DNA fragmentation, caspase-43 activation and poly(ADP-ribose) polymerase (PARP) cleavage, but did not decrease the cell viability of the osteoblast-like cell line MC3T3-E1. MWCNTs also induced loss of the mitochondrial membrane potential (渭m) by regulating expression of Bcl-2 family proteins and caused release of cytochrome c from mitochondria to cytosol. MWCNTs-induced apoptosis in osteoclasts was inhibited both by cyclosporin A, a blocker of the mitochondrial permeability transition pore, and by DEVD-CHO, a cell-permeable inhibitor of caspase-3. The present study suggests that MWCNTs suppresse osteoclastogenesis via the inhibition of osteoclast differentiation and the induction of apoptosis in osteoclasts, rendering them promising candidate for the treatment of osteoclast-related diseases. Copyright 漏 2012 American Scientific Publishers.
语种	英语
出版者	American Scientific Publishers
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/87400]
专题	材料学院－已发表论文
推荐引用方式 GB/T 7714	Ye, Shefang,Jiang, Yuanq,Zhang, Honggang,et al. Multi-walled carbon nanotubes induce apoptosis in RAW 2647 cell-derived osteoclasts through mitochondria-mediated death pathway[J]. http://dx.doi.org/10.1166/jnn.2012.5677,2012.
APA	Ye, Shefang.,Jiang, Yuanq.,Zhang, Honggang.,Wang, Yifang.,Wu, Yihui.,...&张其清.(2012).Multi-walled carbon nanotubes induce apoptosis in RAW 2647 cell-derived osteoclasts through mitochondria-mediated death pathway.http://dx.doi.org/10.1166/jnn.2012.5677.
MLA	Ye, Shefang,et al."Multi-walled carbon nanotubes induce apoptosis in RAW 2647 cell-derived osteoclasts through mitochondria-mediated death pathway".http://dx.doi.org/10.1166/jnn.2012.5677 (2012).