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	Separation of superoxide dismutase by size-exclusion chromatography column packed with regenerated cellulose gels
	Yang, G. ; Xiong, X. P. ; Zhang, L. ; Xiong XP(熊晓鹏)
	2003
关键词	chromatography enzymes separation of polymers fluorescene gels
英文摘要	A preparative size-exclusion chromatography (SEC) column (500 X 10 mm) packed with regenerated cellulose gel particles was used for the separation of superoxide dismutase (SOD) from pig blood. The fraction F-5, with an M-w of 12.4 X 10(4), composed of more aspartic acid (Asp), glutamine (Glu), and tyrosine (Tyr) but less cysteine (Cys), has the highest specific activity (204 units per mg protein), which was higher than that of the commercial product (175 units per mg protein). The change of fluorescence intensities at zero concentration of the fractions was identical to that of the activities. The results from amino acid sequence analysis and size-exclusion chromatography combined with static laser light scattering (SEC-LLS) analysis indicated that the separation mechanism of SOD is based on both size exclusion and ion affinity. They also showed that the activity of SOD is determined mainly by its composition. At a flow rate of 0.62 mL min(-1), 42 mg of a high specific activity product can be obtained from crude SOD in 1 day. (C) 2003 Wiley Periodicals, Inc.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/59676]
专题	材料学院－已发表论文
推荐引用方式 GB/T 7714	Yang, G.,Xiong, X. P.,Zhang, L.,et al. Separation of superoxide dismutase by size-exclusion chromatography column packed with regenerated cellulose gels[J],2003.
APA	Yang, G.,Xiong, X. P.,Zhang, L.,&熊晓鹏.(2003).Separation of superoxide dismutase by size-exclusion chromatography column packed with regenerated cellulose gels..
MLA	Yang, G.,et al."Separation of superoxide dismutase by size-exclusion chromatography column packed with regenerated cellulose gels".(2003).

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