In vitro and in vivo studies on gelatin-siloxane nanoparticles conjugated with SynB peptide to increase drug delivery to the brain

CORC > 厦门大学 > 材料学院－已发表论文

	In vitro and in vivo studies on gelatin-siloxane nanoparticles conjugated with SynB peptide to increase drug delivery to the brain
	Tian, Xin-hua ; Wei, Feng ; Wang, Tian-xiao ; Wang, Peng ; Lin, Xiao-ning ; Wang, Jun ; Wang, Dong ; Ren, Lei ; Ren L(任磊)
刊名	http://dx.doi.org/10.2147/IJN.S26541
	2012
关键词	CELL-PENETRATING PEPTIDES PEG-PLGA NANOPARTICLES QUANTUM DOTS LIPOSOMES MECHANISM EFFICACY VECTOR
英文摘要	National Natural Science Foundation of China [81172394, 30970733]; National Basic Research Program of China [2010CB732402]; Natural Science Foundation of Fujian Province of China [2006J0188]; Background: Nanobiotechnology can provide more efficient tools for diagnosis, targeted and personalized therapy, and increase the chances of brain tumor treatment being successful. Use of nanoparticles is a promising strategy for overcoming the blood-brain barrier and delivering drugs to the brain. Gelatin-siloxane (GS) nanoparticles modified with Tat peptide can enhance plasmid DNA transfection efficiency compared with a commercial reagent. Methods: SynB-PEG-GS nanoparticles are membrane-penetrable, and can cross the blood-brain barrier and deliver a drug to its target site in the brain. The efficiency of delivery was investigated in vivo and in vitro using brain capillary endothelial cells, a cocultured blood-brain barrier model, and a normal mouse model. Results: Our study demonstrated that both SynB-PEG-GS and PEG-GS nanoparticles had a spherical shape and an average diameter of 150-200 nm. It was shown by MTT assay that SynB-PEG-GS nanoparticles had good biocompatibility with brain capillary endothelial cells. Cellular uptake by SynB-PEG-GS nanoparticles was higher than that for PEG-GS nanoparticles for all incubation periods. The amount of SynB-PEG-GS nanoparticles crossing the cocultured blood-brain barrier model was significantly higher than that of PEG-GS nanoparticles at all time points measured (P < 0.05). In animal testing, SynB-PEG-GS nanoparticle levels in the brain were significantly higher than those of PEG-GS nanoparticles at all time points measured (P < 0.01). In contrast with localization in the brain, PEG-GS nanoparticle levels were significantly higher than those of SynB-PEG-GS nanoparticles (P < 0.01) in the liver. Conclusion: This study indicates that SynB-PEG-GS nanoparticles have favorable properties with regard to morphology, size distribution, and toxicity. Moreover, the SynB-PEG-GS nanoparticles exhibited more efficient brain capillary endothelial cell uptake and improved crossing of the blood-brain barrier. Further, biodistribution studies of rhodamine-loaded nanoparticles demonstrated that modification with the SynB peptide could not only improve the ability of PEG-GS nanoparticles to evade capture in the reticuloendothelial system but also enhance their efficiency in crossing the blood-brain barrier.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/59011]
专题	材料学院－已发表论文
推荐引用方式 GB/T 7714	Tian, Xin-hua,Wei, Feng,Wang, Tian-xiao,et al. In vitro and in vivo studies on gelatin-siloxane nanoparticles conjugated with SynB peptide to increase drug delivery to the brain[J]. http://dx.doi.org/10.2147/IJN.S26541,2012.
APA	Tian, Xin-hua.,Wei, Feng.,Wang, Tian-xiao.,Wang, Peng.,Lin, Xiao-ning.,...&任磊.(2012).In vitro and in vivo studies on gelatin-siloxane nanoparticles conjugated with SynB peptide to increase drug delivery to the brain.http://dx.doi.org/10.2147/IJN.S26541.
MLA	Tian, Xin-hua,et al."In vitro and in vivo studies on gelatin-siloxane nanoparticles conjugated with SynB peptide to increase drug delivery to the brain".http://dx.doi.org/10.2147/IJN.S26541 (2012).