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	Genomic organization and tissue-specific expression analysis of hepcidin-like genes from black porgy (Acanthopagrus schlegelii B.)
	Ming Yang ; Yang M(杨明) ; Ke-Jian Wang ; Wang KJ(王克坚) ; Jun-Hui Chen ; Chen JH(陈君慧) ; Hai-Dong Qu ; Qu HD(曲海东) ; Shao-Jing Li ; Li SJ(李少菁)
	2007
关键词	Acanthopagrus schlegelii Black porgy Hepcidin Genomic organization Gene expression
英文摘要	【英文摘要】Hepcidin is an antimicrobial peptide and putative iron regulatory hormone previously described in mice and humans. Dozens of fish hepcidins have been isolated and characterized so far. Here we present seven hepcidin-like cDNA sequences named AS-hepc1–7, amplified from the normal commercially cultured fish (black porgy) by RACE–PCR. Sequence analysis reveals that these seven potential hepcidin peptides have highly conserved sequences with other known hepcidins, but they are different from each other in constitution and characteristics of predicted mature amino acids. Based on the study, it is deduced that AS-hepc1–7 represent different variants of a family of hepcidin genes in black porgy. To understand the organization of these hepcidin-like genes, we sequenced AS-hepc2 DNA, AS-hepc3 DNA, AS-hepc4 DNA, AS-hepc7 DNA and AS-hepc2 upstream region; and all of the four genomic DNAs consisted of two introns and three exons, the same organization as other reported hepcidins. The tissue-specific gene expression of hepcidins in normal black porgy was evaluated using RT–PCR and dot blot approaches. RT–PCR showed that transcripts of hepcidin-like mRNAs were present in each tested tissue of normal juvenile black porgy, including liver, spleen, kidney, heart, brain, stomach, intestine, gill, skin and blood, but abundant hepcidin-like mRNA transcripts were only detected in the liver, kidney, spleen, intestine and stomach by dot blot assay. In addition, using dot blot and Northern blot approach, a significant increase of hepcidin mRNA transcription was observed in the liver within 48 h after immersion in a suspension of live bacteria, which suggested that the expression pattern of hepcidin-like genes in black porgy might be different in the liver from the other tissues as previously reported in several hepcidin studies.
语种	英语
出版者	《Fish & Shellfish Immunology》编辑部
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/1656]
专题	李少菁 海洋环境－已发表论文
推荐引用方式 GB/T 7714	Ming Yang,Yang M,Ke-Jian Wang,et al. Genomic organization and tissue-specific expression analysis of hepcidin-like genes from black porgy (Acanthopagrus schlegelii B.)[J],2007.
APA	Ming Yang.,杨明.,Ke-Jian Wang.,王克坚.,Jun-Hui Chen.,...&李少菁.(2007).Genomic organization and tissue-specific expression analysis of hepcidin-like genes from black porgy (Acanthopagrus schlegelii B.)..
MLA	Ming Yang,et al."Genomic organization and tissue-specific expression analysis of hepcidin-like genes from black porgy (Acanthopagrus schlegelii B.)".(2007).

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