改进的流动注射催化光度法测定啤酒中微量甲醛

CORC > 集美大学

	改进的流动注射催化光度法测定啤酒中微量甲醛
	陈婷 ; 黄志勇 ; 李连平 ; 戴玉雷
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=fxhx200803023&dbcode=CJFQ&dbname=CJFQ2008
	2012-06-05 ; 2012-06-05
关键词	催化光度法流动注射甲醛啤酒 Kinetic spectrophotometry flow injection formaldehyde beer TS262.5
其他题名	Determination of Formaldehyde in Beers by Flow Injection Coupled with Kinetic Spectrophotometry
中文摘要	甲醛对KBrO3氧化结晶紫的催化作用可用来测量甲醛的含量,但该反应往往需要较高温度才能有效进行。当采用流动注射光度法测量时,加热产生的气泡使管路液体不连续。本实验通过自制的三通管,借助蠕动泵可将气泡在线地抽出而保证管路不断流,并将此方法用于啤酒中微量甲醛含量的测定。经优化发现,当V(1.0mol/LH3PO4)∶V(0.04mo1/LKBrO3)=1∶2的混合溶液与3.5×10-4mol/L结晶紫反应时,在90℃温度下反应60s可使催化体系和非催化体系的吸光度差值(ΔA)达到最大。甲醛浓度在0.05～2mg/L范围内与ΔA值有良好的线性关系(r>0.999);检出限为2.49μg/L;加标回收率为93.3%～102%。所测的5种不同品牌的啤酒样品中甲醛含量均低于发酵酒卫生标准(GB/T5009.49-2003)中的相关规定值。; When the ordinary flow injection analytical method based on the oxidation of crystal violet by potassium bromate in the phosphoric acid medium was used for the determination of trace formaldehyde,it encounters the problem of a higher temperature bubbles emerge in the reaction tube.In the present experiment,a de-bubbling device by home-made three-way joint(T-piece)was developed in this kinetic spectrophotometric method coupled with flow injection.The trace formaldehyde in beers after being distillated could be measured.The results indicated that the most absorbance difference between the catalyst and non-catalyst systems could be obtained when the mixture of 1.0 mol/L of phosphoric acid and 0.04 mo1/L of bromate at 1∶2(v/v)reacted with 3.5×10-4 mol/L of crystal violet at the temperature of 90 ℃ for 60 s.Under the optimization conditions,linear response of formaldehyde ranged from 0.05 to 2 mg/L(r>0.999).The detection limit measured with 11 replicates of procedure blank and estimated with 3-fold standard deviation was 2.49 μg/L.The recovery tests by standard additions ranged from 93.3% to 102%.The formaldehyde content in the five kinds of beers measured was lower than the limit of hygienic standard of fermented wines of China(GB/T 5009.49-2003).The method was suitable for the measurement of trace formaldehyde in beers.; 【作者单位】集美大学生物工程学院；集美大学生物工程学院厦门361021；厦门361021；【作者英文名】Chen Ting,Huang Zhi-Yong,Li Lian-Ping,Dai Yu-Lei(College of Bioengineering,Jimei University,Xiamen 361021)
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/18764]
专题	集美大学
推荐引用方式 GB/T 7714	陈婷,黄志勇,李连平,等. 改进的流动注射催化光度法测定啤酒中微量甲醛[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=fxhx200803023&dbcode=CJFQ&dbname=CJFQ2008,2012, 2012.
APA	陈婷,黄志勇,李连平,&戴玉雷.(2012).改进的流动注射催化光度法测定啤酒中微量甲醛.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=fxhx200803023&dbcode=CJFQ&dbname=CJFQ2008.
MLA	陈婷,et al."改进的流动注射催化光度法测定啤酒中微量甲醛".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=fxhx200803023&dbcode=CJFQ&dbname=CJFQ2008 (2012).