鲤鱼组织中氯霉素的代谢速率及其色谱-质谱的表征方法研究

CORC > 集美大学

	鲤鱼组织中氯霉素的代谢速率及其色谱-质谱的表征方法研究
	黄志勇 ; 沈金灿 ; 彭爱红 ; 孙茂营 ; 庄峙厦 ; 王小如
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=zgsp200605015&dbcode=CJFQ&dbname=CJFQ2006
	2012-06-05 ; 2012-06-05
关键词	氯霉素色谱-质谱残留 Chloraphenicol HPLC-ESI-MS Residue TS254
其他题名	Studies on the Metabolic Rate of Chloraphenicol in Cyprinus Carpio Linnaeus and Its Characterization by HPLC-ESI-MS
中文摘要	目的:研究氯霉素在鲤鱼体内的药物代谢动力学规律,并用色谱-质谱方法对代谢过程中残留的氯霉素进行结构表征。方法:以鲤鱼为实验动物,按80mg/kgbw剂量进行背部肌肉注射,用药代动力学参数描述氯霉素在鱼体内的代谢消除行为;在优化的反相色谱与电喷雾电离质谱(HPLC-ESI-MS)联用系统条件下,采用负离子检测模式,对鱼肌肉氯霉素进行结构分析。结果:氯霉素在鱼肌肉的残留呈现多峰特点,其消除半衰期为17.81h。同时,通过HPLC-ESI-MS联用技术,可清晰地获得氯霉素的母离子峰和相关的碎片离子信息。结论:氯霉素在鲤鱼体内具有较长的残留时间,HPLC-ESI-MS联用技术可用于水产样品中氯霉素残留的确证分析。; Objective:To investigate the elimination of chloramphenicol(CAP)in Cyprinus Carpio Linnaeus and characterize the structure of CAP with the high performance liquid chromatography coupled with electrospray ionization mass spectrometry(HPLC-ESI-MS). Methods:Using Cyprinus Carpio Linnaeus as experimental animal. after single injection with 80 mg/kg bw of CAP,the pharmacokinetic parameters were used to describe the elimination behaviors of CAP in tissues. And structure of CAP was analyzed with the optimized hyphenated technique of HPLC-ESI-MS. Results:The distribution of CAP in muscle was presented as multi-peaks and the elimination half-life(t1/2)of CAP in muscle was 17.81 h. The molecular ions with different isotopes and transitions ions of CAP were monitored with ESI-MS at the negative detection mode. Conclusion:The CAP might remain a long period of time in fish tissues. The residue of CAP in aquatic products can be qualitatively determined with HPLC-ESI-MS.; 【作者单位】集美大学生物工程学院；厦门大学化学系现代分析科学教育部重点实验室；国家海洋局第一研究所厦门361021；厦门361005；厦门361021；青岛266061；【作者英文名】Huang Zhiyong1 Shen Jincan3 Peng Aihong1 Sun Maoying1 Zhuang Zhixia3 Wang Xiaoru2 (1College of Bioengineering,Jimei University,Xiamen 361021 2First Institute Oceanorgraphy,S.O.A,Qingdao 266061 3The Key Laboratory of Analytical Science of Ministry of
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/18749]
专题	集美大学
推荐引用方式 GB/T 7714	黄志勇,沈金灿,彭爱红,等. 鲤鱼组织中氯霉素的代谢速率及其色谱-质谱的表征方法研究[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=zgsp200605015&dbcode=CJFQ&dbname=CJFQ2006,2012, 2012.
APA	黄志勇,沈金灿,彭爱红,孙茂营,庄峙厦,&王小如.(2012).鲤鱼组织中氯霉素的代谢速率及其色谱-质谱的表征方法研究.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=zgsp200605015&dbcode=CJFQ&dbname=CJFQ2006.
MLA	黄志勇,et al."鲤鱼组织中氯霉素的代谢速率及其色谱-质谱的表征方法研究".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=zgsp200605015&dbcode=CJFQ&dbname=CJFQ2006 (2012).