柱前衍生高效液相色谱法测定食品中的氨基酸含量

CORC > 集美大学

	柱前衍生高效液相色谱法测定食品中的氨基酸含量
	吴光斌 ; 陈发河
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=znjx200603007&dbcode=CJFQ&dbname=CJFQ2006
	2012-06-05 ; 2012-06-05
关键词	6-氨基喹啉基-N-羟基琥珀酰亚胺基甲酸酯高效液相色谱法柱前衍生氨基酸 6-aminoquinoly-N-hydroxysuccinimidy carbamate High Performance Liquid Chromatography(HPLC) precolumn derivatization amino acids TS207.3 O657.72
其他题名	Determination of amino acids in food by High Performance Liquid Chromatography with precolumn derivatization
中文摘要	采用柱前衍生高效液相色谱法,测定了3种食品中天冬氨酸、赖氨酸、亮氨酸等17种氨基酸的含量.以6-氨基喹啉基-N-羟基琥珀酰亚胺基甲酸酯(AQC)为衍生试剂,醋酸盐-磷酸盐缓冲液、乙睛、水为流动相,梯度洗脱.AccQ.Tag(Waters)氨基酸分析柱为色谱柱,紫外检测.结果显示,氨基酸浓度与峰面积呈良好的线性关系,线性范围10~80μmol/L,最小检出限为0.23μmol/L,相关系数均在0.998以上,相对标准偏差在1.8%~4.9%之间,回收率在89.3%~115.8%之间.; The technology of precolumn derivatization,along with High Performance Liquid Chromatography gradient elution method was used in determining contents of amino acids in food.A high-efficiency AccQ·Tag(Waters) was used as chromatographic column,and acetate-phosphate buffer,acetonitrile and twice distilled water were used as mobile phase during gradient elution.AQC(6-aminoquinoly-N-hydroxysuccinimidy carbamate),a derivation reagent,was used for sample derivatization.Conditions of the test were column temperature 37℃,wavelength 248 nm and flow rate(1.0 mL/min).The results showed that concentration of amino acids and peak areas achieved a nice linear relations(r is higher than 0.998) with linear range 10～80 μmol/L.RSD were 1.8%～4.9%.The limit of detection is 0.23 μmol/L.The ranges of recovery of amino acids are from 89.3%～115.8%.; 【作者单位】集美大学生物工程学院；集美大学生物工程学院福建厦门361021；福建厦门361021；【作者英文名】WU Guang-bin,CHEN Fa-he(College of Bio-engineering,Jimei University,Xiamen 361021,China)
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/18649]
专题	集美大学
推荐引用方式 GB/T 7714	吴光斌,陈发河. 柱前衍生高效液相色谱法测定食品中的氨基酸含量[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=znjx200603007&dbcode=CJFQ&dbname=CJFQ2006,2012, 2012.
APA	吴光斌,&陈发河.(2012).柱前衍生高效液相色谱法测定食品中的氨基酸含量.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=znjx200603007&dbcode=CJFQ&dbname=CJFQ2006.
MLA	吴光斌,et al."柱前衍生高效液相色谱法测定食品中的氨基酸含量".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=znjx200603007&dbcode=CJFQ&dbname=CJFQ2006 (2012).