紫外分光光度法测定发酵液中麦考酚酸含量

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	紫外分光光度法测定发酵液中麦考酚酸含量
	陈俊 ; 肖安风 ; 倪辉 ; 杨秋明 ; 蔡慧农
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz201104005&dbcode=CJFQ&dbname=CJFQ2011
	2012-06-05 ; 2012-06-05
关键词	短密青霉菌麦考酚酸紫外分光光度法发酵液 Penicillium brevicompactum mycophenolic acid UV spectrophotometeric method fermentation broth R927.2
其他题名	Determination of Mycophenolic Acid in Fermentation Broth by UV Spectrophotometric Method
中文摘要	分别采用乙醇、乙酸乙酯、甲醇3种有机溶剂提取发酵液中的麦考酚酸(MPA),分析紫外光谱吸收特征,并建立了测定MPA含量的紫外分光光度法.结果表明:MPA甲醇溶液质量浓度在0～50 mg/L范围内与Abs304呈良好的线性关系,平均回收率为101.8%,相对标准偏差为1.20%.紫外分光光度法测定MPA含量简单、快速、重复性好,适用于发酵液中MPA含量的准确测定.; A UV spectrophotometric method to determinine mycophenolic acid in zymotic fluid using ethanol,ethylacetate and methanol as extraction was established.The result showed:the linearity was in the range of 0～50 mg/L of MPA methanol solution,the average recovery was 101.8 %,and RSD was 1.20 %.The method was simple,rapid and reproducible,and it was suitable for the determination of mycophenolic acid in fermentation broth.; 【作者单位】华侨大学化工学院；集美大学生物工程学院；厦门市食品生物工程技术研究中心；【作者英文名】CHEN Jun1,2,XIAO An-feng2,3,NI Hui2,3,YANG Qiu-ming2,3,CAI Hui-nong2,3(1.College of Chemical Engineering,Huaqiao University,Xiamen 361021,China;2.School of Biotechnology Engineering,Jimei University,Xiamen 361021,China;3.Food and Bio-engineering Tech
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/18634]
专题	集美大学
推荐引用方式 GB/T 7714	陈俊,肖安风,倪辉,等. 紫外分光光度法测定发酵液中麦考酚酸含量[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz201104005&dbcode=CJFQ&dbname=CJFQ2011,2012, 2012.
APA	陈俊,肖安风,倪辉,杨秋明,&蔡慧农.(2012).紫外分光光度法测定发酵液中麦考酚酸含量.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz201104005&dbcode=CJFQ&dbname=CJFQ2011.
MLA	陈俊,et al."紫外分光光度法测定发酵液中麦考酚酸含量".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz201104005&dbcode=CJFQ&dbname=CJFQ2011 (2012).