紫外分光光度法测定发酵液中麦考酚酸含量 | |
陈俊 ; 肖安风 ; 倪辉 ; 杨秋明 ; 蔡慧农 | |
刊名 | http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz201104005&dbcode=CJFQ&dbname=CJFQ2011
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2012-06-05 ; 2012-06-05 | |
关键词 | 短密青霉菌 麦考酚酸 紫外分光光度法 发酵液 Penicillium brevicompactum mycophenolic acid UV spectrophotometeric method fermentation broth R927.2 |
其他题名 | Determination of Mycophenolic Acid in Fermentation Broth by UV Spectrophotometric Method |
中文摘要 | 分别采用乙醇、乙酸乙酯、甲醇3种有机溶剂提取发酵液中的麦考酚酸(MPA),分析紫外光谱吸收特征,并建立了测定MPA含量的紫外分光光度法.结果表明:MPA甲醇溶液质量浓度在0~50 mg/L范围内与Abs304呈良好的线性关系,平均回收率为101.8%,相对标准偏差为1.20%.紫外分光光度法测定MPA含量简单、快速、重复性好,适用于发酵液中MPA含量的准确测定.; A UV spectrophotometric method to determinine mycophenolic acid in zymotic fluid using ethanol,ethylacetate and methanol as extraction was established.The result showed:the linearity was in the range of 0~50 mg/L of MPA methanol solution,the average recovery was 101.8 %,and RSD was 1.20 %.The method was simple,rapid and reproducible,and it was suitable for the determination of mycophenolic acid in fermentation broth.; 【作者单位】华侨大学化工学院; 集美大学生物工程学院; 厦门市食品生物工程技术研究中心;【作者英文名】CHEN Jun1,2,XIAO An-feng2,3,NI Hui2,3,YANG Qiu-ming2,3,CAI Hui-nong2,3(1.College of Chemical Engineering,Huaqiao University,Xiamen 361021,China;2.School of Biotechnology Engineering,Jimei University,Xiamen 361021,China;3.Food and Bio-engineering Tech |
语种 | 中文 |
内容类型 | 期刊论文 |
源URL | [http://ir.calis.edu.cn/hdl/235041/18634] ![]() |
专题 | 集美大学 |
推荐引用方式 GB/T 7714 | 陈俊,肖安风,倪辉,等. 紫外分光光度法测定发酵液中麦考酚酸含量[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz201104005&dbcode=CJFQ&dbname=CJFQ2011,2012, 2012. |
APA | 陈俊,肖安风,倪辉,杨秋明,&蔡慧农.(2012).紫外分光光度法测定发酵液中麦考酚酸含量.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz201104005&dbcode=CJFQ&dbname=CJFQ2011. |
MLA | 陈俊,et al."紫外分光光度法测定发酵液中麦考酚酸含量".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz201104005&dbcode=CJFQ&dbname=CJFQ2011 (2012). |
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