毛细管电泳-电化学发光法分离检测饮料中亮氨酸

CORC > 集美大学

	毛细管电泳-电化学发光法分离检测饮料中亮氨酸
	冯珍鸽 ; 蔡慧农 ; 王力
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=FZKB2011092800D&dbcode=CJFQ&dbname=CAPJ
	2012-06-05 ; 2012-06-05
关键词	毛细管电泳电化学发光三联吡啶钌亮氨酸饮料 Capillary electrophoresis Electrochemiluminescence Ru(bpy)32+ Leucine Beverage O657
其他题名	Separation and Determination of Leucine in Beverages by Capillary Electrophoresis with Electrochemiluminesence Detection
中文摘要	基于亮氨酸对化学发光试剂三联吡啶钌(Ru(bpy)32+)在铂电极上电致发光信号的增敏作用,建立了一种快速测定亮氨酸的毛细管电泳-电化学发光分析方法。对实验条件进行优化,最佳实验条件如下:检测电位1.15V;Ru(bpy)32+浓度7mmolL-1(pH=8.5);进样时间10s;进样高压10kV;分离高压14kV;运行缓冲液pH8.5,20mmolL-1磷酸盐缓冲液。在最佳实验条件下,6min内可实现亮氨酸的分离检测,线性范围2～320μgmL-1(R=0.9998),最低检出限(S/N=3)为0.13μgmL-1。对饮料样品进行测定,峰高和迁移时间的相对标准偏差(R.S.D.)分别为1.94%和0.50%(n=5)。该方法具有分析速度快、分离效果好、灵敏度高、重现性好等特点,适用于饮料中亮氨酸的快速检测。; A new sensitive method is described for determination of Leucine in beverages by capillary electrophoresis with an electrochemiluminescence detection (CE-ECL) based on tris(2,2’-bipyridyl)ruthenium(II) [Ru(bpy) 3 2+ ]. Experimental parameters influencing the effects of separation and determination were explored in details. Under the optimal conditions, such as detection potential at 1.15V, 7mmol L -1 Ru(bpy)32+ (pH=8.5), sample injection at 10s×10kV, separation voltage at 14kV, 20mmol L -1 of phosphate buffer at pH 8.5, the determination of Leucine was accomplished within 6min. The ECL intensity was linear with the concentration of Leucine in the range of 2～320μg mL -1(R=0.9998) with the detection limit (S/N=3) of 0.13μg mL -1 . The relative standard deviations (R.S.D.) of ECL intensity and migration time for five injections of beverage sample were 1.94% and 0.50% (n=5), respectively. This method was successfully used to determination of Leucine in beverage with satisfactory results.; 【作者单位】集美大学生物工程学院；【作者英文名】Feng Zhen-ge, Cai Hui-nong, Wang Li* (Bioengineering College of Jimei University, Xiamen 361021, Fujian)
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/18526]
专题	集美大学
推荐引用方式 GB/T 7714	冯珍鸽,蔡慧农,王力. 毛细管电泳-电化学发光法分离检测饮料中亮氨酸[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=FZKB2011092800D&dbcode=CJFQ&dbname=CAPJ,2012, 2012.
APA	冯珍鸽,蔡慧农,&王力.(2012).毛细管电泳-电化学发光法分离检测饮料中亮氨酸.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=FZKB2011092800D&dbcode=CJFQ&dbname=CAPJ.
MLA	冯珍鸽,et al."毛细管电泳-电化学发光法分离检测饮料中亮氨酸".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=FZKB2011092800D&dbcode=CJFQ&dbname=CAPJ (2012).