毛细管电泳-电化学发光法分离检测饮料中亮氨酸

CORC > 集美大学

	毛细管电泳-电化学发光法分离检测饮料中亮氨酸
	蔡慧农 ; 骆聪婷 ; 王力
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=fzkb201106003&dbcode=CJFQ&dbname=CJFQ2011
	2012-06-05 ; 2012-06-05
关键词	毛细管电泳电化学发光三联吡啶钌亮氨酸饮料 capillary electrophoresis electrochemiluminescence Ru(bpy)32+ leucine beverage TS27 O658.9
其他题名	Separation and determination of leucine in beverages by capillary electrophoresis with electrochemiluminesence detection
中文摘要	基于亮氨酸对化学发光试剂三联吡啶钌(Ru(bpy)32+)在铂电极上电致发光信号的增敏作用,建立了一种快速测定亮氨酸的毛细管电泳-电化学发光分析方法.对实验条件进行优化,最佳实验条件如下:检测电位为1.15 V;Ru(bpy)32+浓度为7 mmol.L-1(pH=8.5);进样时间为10 s;进样高压为10 kv;分离高压为14 kv;运行缓冲液pH=8.5,20 mmol.L-1磷酸盐缓冲液.在最佳实验条件下,6 min内可实现亮氨酸的分离检测,线性范围2～320 mg.L-1(R=0.999 8),最低检出限(S/N=3)为0.13 mg.L-1.对饮料样品进行测定,峰高和迁移时间的相对标准偏差(RSD)分别为1.94%和0.50%(n=5).该方法具有分析速度快、分离效果好、灵敏度高、重现性好等特点,适用于饮料中亮氨酸的快速检测.; A new sensitive method is described for determination of leucine in beverages by capillary electrophoresis with an electrochemiluminescence detection(CE-ECL) based on tris(2,2′-bipyridyl)ruthenium(Ⅱ) [Ru(bpy)32+].Experimental parameters influencing the effects of separation and determination were explored in details.Under the optimal conditions,such as detection potential at 1.15 V,7 mmol·L-1 Ru(bpy)32+(pH=8.5),sample injection at 10 s×10 kV,separation voltage at 14 kV,20 mmol·L-1 of phosphate buffer at pH=8.5,the determination of leucine was accomplished within 6min.The ECL intensity was linear with the concentration of leucine in the range of 2～320 mg·L-1(R=0.999 8) with the detection limit(S/N=3) of 0.13 mg·L-1.The relative standard deviations(RSD) of ECL intensity and migration time for five injections of beverage sample were 1.94% and 0.50%(n=5),respectively.This method was successfully used to determination of Leucine in beverage with satisfactory results.; 【作者单位】集美大学生物工程学院；【作者英文名】CAI Hui-nong,LUO Cong-ting,WANG Li(Bioengineering College of Jimei University,Xiamen 361021,China)
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/18524]
专题	集美大学
推荐引用方式 GB/T 7714	蔡慧农,骆聪婷,王力. 毛细管电泳-电化学发光法分离检测饮料中亮氨酸[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=fzkb201106003&dbcode=CJFQ&dbname=CJFQ2011,2012, 2012.
APA	蔡慧农,骆聪婷,&王力.(2012).毛细管电泳-电化学发光法分离检测饮料中亮氨酸.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=fzkb201106003&dbcode=CJFQ&dbname=CJFQ2011.
MLA	蔡慧农,et al."毛细管电泳-电化学发光法分离检测饮料中亮氨酸".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=fzkb201106003&dbcode=CJFQ&dbname=CJFQ2011 (2012).