烟管菌漆酶合成的营养条件研究

CORC > 集美大学

	烟管菌漆酶合成的营养条件研究
	王剑锋 ; 苏国成 ; 刘建玲 ; 王璋
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=spfx200602009&dbcode=CJFQ&dbname=CJFQ2006
	2012-06-05 ; 2012-06-05
关键词	烟管菌漆酶营养条件 Bjerkandera adusta laccase nutrional factors TS201.3
其他题名	Effects of Nutritional Factors on Laccase Production from White Rot Fungi Bjerkandera adusta
中文摘要	对筛选到的漆酶高效产生菌烟管菌（Bjerkandera adusta）WZFF．W-Y11合成漆酶的营养条件进行了研究,发现该烟管菌漆酶属于一种组成型酶,酶活力高,产酶高峰到来早,酶的合成与菌体生长过程紧密相关。漆酶合成需要丰富的营养因子,麸皮水解液是重要的成分,葡萄糖-淀粉的复合碳源和氯化铵-豆饼粉的复合氮源以及适量的无机盐有利于漆酶的产生。虽然增加 Fe2+的量明显抑制了漆酶的产生,但 Cu2+却能有效地促进漆酶的合成。在经过优化的培养条件下,酶活力高达1 108 U/mL。; The nutritional conditions in liquid culture medium for the laccase production from a novel isolated strain Bjerkandera adusta WZFF.W-Yll were studied.The fungal laccase was found to be a kind of composite enzyme with high activity.The enzyme production is closely related with mycelium growth and biomass and the maximal enzyme productivity could be reached within a short period.The effects of several nutritional factors on production of the enzyme were investigated,and the results showed that wheat bran was an important component for laccase production.Using glucose and soluble starch as carbon sources,ammonium chloride and powder of bean cake as nitrogen sources and adding suitable inorganic salts,a high activity level was obtained in this study. Though ferric ion showed significant inhibition effect on laccase biosynthesis,Cu2+could effectively enhance the enzyme production.Under the optimized nutritional culture condition,the enzyme activity reached as high as to 1108 U/mL.; 【作者单位】西北大学生命科学学院； 3 集美大学生物工程学院；中国食品发酵工业研究院西安； 710069中国食品发酵工业研究院；北京； 100027 硕士研究生；厦门； 361201；西安； 710069； 100027；【作者英文名】Wang Jianfeng~(1,2) Su Guocheng~3 Liu Jianling~1 Wang Zhang~2 1(College of Life Science,Northwest University,Xi’an 710069,China) 2(China National Institute of Food and Fermentation Industries,Beijing 100027,China) 3(School of Biotechnology,Jimei Uni
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/18491]
专题	集美大学
推荐引用方式 GB/T 7714	王剑锋,苏国成,刘建玲,等. 烟管菌漆酶合成的营养条件研究[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=spfx200602009&dbcode=CJFQ&dbname=CJFQ2006,2012, 2012.
APA	王剑锋,苏国成,刘建玲,&王璋.(2012).烟管菌漆酶合成的营养条件研究.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=spfx200602009&dbcode=CJFQ&dbname=CJFQ2006.
MLA	王剑锋,et al."烟管菌漆酶合成的营养条件研究".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=spfx200602009&dbcode=CJFQ&dbname=CJFQ2006 (2012).