酸性蛋白酶水解鳗鱼工艺条件的优化

CORC > 集美大学

	酸性蛋白酶水解鳗鱼工艺条件的优化
	杨远帆 ; 倪辉 ; 陈申如 ; 黄志勇 ; 蔡慧农
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=fjnd201103018&dbcode=CJFQ&dbname=CJFQ2011
	2012-06-05 ; 2012-06-05
关键词	鳗鱼酸性蛋白酶水解优化 eel acid protease hydrolysis optimization TS254.4
其他题名	Optimal conditions for the hydrolyzation of eel with acid protease
中文摘要	本研究通过单因素试验及响应面分析试验优化了酸性蛋白酶水解鳗鱼的最优条件:加酶量19766 U.g-1,底物蛋白浓度15.28%,水解温度45℃,pH 3.0.在此条件下,可通过改变水解时间来控制水解度,从而制备不同工艺要求的水解产物.当水解时间为8 h时,水解反应达到平衡,水解度为33.35%,总氮回收率为80.63%.; The effects of the main parameters including hydrolysis temperature,pH value,hydrolysis time were investigated with single factor experiment and dosage of enzyme,protein concentration were investigated with response surface methodology(RSM).The optimum enzymatic hydrolysis conditions were as follows: the protein substrate with a final concentration of 15.28% was mixed with acid protease with a final concentration of 19766 U per gram of protein at pH 3.0.After incubating at 45 ℃ for 8 h,the degree of hydrolysis was 33.35%,and the recovery of total nitrogen was 80.63%.; 【作者单位】集美大学生物工程学院；【作者英文名】YANG Yuan-fan,NI Hui,CHEN Shen-ru,HUANG Zhi-yong,CAI Hui-nong(School of Biotechnology Engineering,Jimei University,Xiamen,Fujian 361021,China)
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/18361]
专题	集美大学
推荐引用方式 GB/T 7714	杨远帆,倪辉,陈申如,等. 酸性蛋白酶水解鳗鱼工艺条件的优化[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=fjnd201103018&dbcode=CJFQ&dbname=CJFQ2011,2012, 2012.
APA	杨远帆,倪辉,陈申如,黄志勇,&蔡慧农.(2012).酸性蛋白酶水解鳗鱼工艺条件的优化.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=fjnd201103018&dbcode=CJFQ&dbname=CJFQ2011.
MLA	杨远帆,et al."酸性蛋白酶水解鳗鱼工艺条件的优化".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=fjnd201103018&dbcode=CJFQ&dbname=CJFQ2011 (2012).