海洋放线菌菌株N350产生的脂溶性抗肿瘤化合物的研究

CORC > 集美大学

	海洋放线菌菌株N350产生的脂溶性抗肿瘤化合物的研究
	曾伟 ; 邵志宇 ; 徐庆妍 ; 郑忠辉 ; 黄耀坚 ; 郭跃伟 ; 苏文金
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=seac2003s2022&dbcode=CJFQ&dbname=CJFQ2003
	2012-06-05 ; 2012-06-05
关键词	海洋放线菌抗肿瘤反式-13-二十二碳单烯酰胺 7,4’-二羟基异黄酮 6-甲氧基-7,4’-二羟基异黄酮 β-胡萝卜甙 marine actinomycetes antitumor cis-13-docosenmamide 7, 4′-dihydroxyisoflavone 6-methoxy-7 4′-dihydroxyisoflavone β-sitosterol-β-D-glucopyranoside R73-36
其他题名	Isolation and structure elucidation of lipophilic cytotoxic compounds produced by a marine actinomycetes, N350
中文摘要	海洋微生物是当前抗肿瘤药物开发的重要新资源.应用硅胶柱层析结合活性跟踪分离的方法,从海洋放线菌菌株N350的脂溶性发酵产物中纯化并鉴定了4个化合物,分别为反式-13-二十二碳单烯酰胺,7,4’-二羟基异黄酮、6-甲氧基-7,4’-二羟基异黄酮及β-胡萝卜甙.其中,反式-13-二十二碳单烯酰胺、7,4’-二羟基异黄酮及6-甲氧基-7,4’-二羟基异黄酮具有抗肿瘤活性,抑制人口腔上皮癌KB细胞生长的ID50分别为15,8 1,5 2μg/cm3,抑制人白血病细胞HL-60生长的ID50分别为7,2 8,1 9μg/cm3.这些化合物均首次在海洋放线菌中得到分离.; Marine microorganism, which can be easily reproduced in large scale in industry, is being exploited for new antitumor drugs with unique structure and excellent bioactivity. Four lipophilic cytotoxic compounds produced by a marine actinomycetes, strain N350, were purified by bioassay-guided isolation, and identified as cis-13-docosenmamide, 7, 4′-dihydroxyisoflavone, 6-methoxy-7, 4′-dihydroxyisoflavone, and β-sitosterol-β-D-glucopyranoside, respectively. Cis-13-docosenamide, 7, 4′-dihydroxyisoflavone and 6-methoxy-7, 4′-dihydroxyisoflavone demonstrated significant cytotoxic activity in vitro. Against human leukemia cell line, HL-60, their IC50 was 2.8, 1.9, 7 μg/cm3, respectively, and against human epidermal carcinoma of oral cavity, KB cell line, IC50 was 8.1, 5.2, 15 μg/cm3, respectively. All these compounds were, for the first time, isolated from the products of marine actinomycetes.; 【作者单位】厦门大学生命科学学院；中国科学院上海药物研究所；厦门大学生命科学学院福建厦门361005；上海200031；福建厦门361005；集美大学生物工程学院；福建厦门361021；【作者英文名】ZENG Wei~1,SHAO Zhi-yu~2,XU Qing-yan~1,ZHENG Zhong-hui~1,HUANG Yao-jian~1,GUO Yue-wei~2,SU Wen-jin~(1,3)(1.School of Life Sciences, Xiamen University, Xiamen 361005,China; 2.Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 200031, C
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/18324]
专题	集美大学
推荐引用方式 GB/T 7714	曾伟,邵志宇,徐庆妍,等. 海洋放线菌菌株N350产生的脂溶性抗肿瘤化合物的研究[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=seac2003s2022&dbcode=CJFQ&dbname=CJFQ2003,2012, 2012.
APA	曾伟.,邵志宇.,徐庆妍.,郑忠辉.,黄耀坚.,...&苏文金.(2012).海洋放线菌菌株N350产生的脂溶性抗肿瘤化合物的研究.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=seac2003s2022&dbcode=CJFQ&dbname=CJFQ2003.
MLA	曾伟,et al."海洋放线菌菌株N350产生的脂溶性抗肿瘤化合物的研究".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=seac2003s2022&dbcode=CJFQ&dbname=CJFQ2003 (2012).