文蛤特异性过敏原免疫识别的初步研究

CORC > 集美大学

	文蛤特异性过敏原免疫识别的初步研究
	刘光明 ; 曹敏杰 ; 梁银龙 ; 蔡朝辉 ; 苏文金
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=spkx200901051&dbcode=CJFQ&dbname=CJFQ2009
	2012-06-05 ; 2012-06-05
关键词	文蛤特异性过敏原免疫杂交聚丙烯酰胺凝胶电泳调查 Meretrix meretrix Linnaeus specific allergen Western blotting SDS-PAGE survey R392
其他题名	Pilot Study on Immunoassay of Specific Allergen of Meretrix meretrix Linnaeus
中文摘要	目的:贝类是人类最优质的食用蛋白资源之一,也是联合国粮农组织公布的八大类过敏食物之一,贝类过敏反应严重影响着过敏人群的身体健康和生活质量,为此开展贝类过敏原的识别检测研究很有必要。方法:通过问卷调查初步了解食物过敏现状,获取自诉贝类过敏患者血清和正常人阴性对照血清,采用特异性IgE检测试剂盒筛选贝类过敏血清,应用组织捣碎提取蛋白、聚丙烯酰胺凝胶电泳和免疫印迹等实验方法研究文蛤特异性过敏原。结果:文蛤肌肉主要蛋白的相对分子量约为200kD以上、200、90、80、70、46、36和24kD,其中能被贝类过敏血清特异性识别的90kD组分约占文蛤肌肉总蛋白的10%～20%,且主要为盐溶性蛋白。结论:文蛤的特异性过敏原为90kD蛋白组分。; Objective:Shellfish is one of the best edible proteins for human.It is also commonly identified as a source of food hypersensitivity,and ingestion of shellfish may cause severe allergic reactions such as anaphylactic shock.The potential risk of shellfish allergy is worth paying more attentions.Method:Meretrix meretrix Linnaeus was selected as the material,and the muscle protein was extracted with a triturator.Nine cases of shellfish-allergic serum and six cases of normal serum were obtained through questionnaire survey and identified with allergen kit.SDS-PAGE and Western blotting were applied to identify the components and characterizations of specific allergen of Meretrix meretrix Linnaeus.Results:There are eight sections of protein with relative molecular weight of more than 200 kD,200,90,80,70,46,36,and 24 kD in SDS-PAGE,respectively.Eight cases of shellfish- allergic serum show IgE-binding capacity to the protein with relative molecular weight of 90 kD in Western blotting.The 90-kD protein accounts for 10% to 20% of the muscle protein of Meretrix meretrix Linnaeus,and mainly is salt-soluble protein. Conclusion:The specific allergenic component in Meretrix meretrix Linnaeus is identified as a 90-kD protein.; 【作者单位】集美大学生物工程学院福建省高校水产科学技术与食品安全重点实验室；【作者英文名】LIU Guang-ming CAO Min-jie LIANG Yin-long CAI Chao-hui SU Wen-jin (College of Biological Engineering,Key Laboratory of Science and Technology for Aquaculture and Food Safety,Jimei University,Xiamen 361021,China)
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/18197]
专题	集美大学
推荐引用方式 GB/T 7714	刘光明,曹敏杰,梁银龙,等. 文蛤特异性过敏原免疫识别的初步研究[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=spkx200901051&dbcode=CJFQ&dbname=CJFQ2009,2012, 2012.
APA	刘光明,曹敏杰,梁银龙,蔡朝辉,&苏文金.(2012).文蛤特异性过敏原免疫识别的初步研究.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=spkx200901051&dbcode=CJFQ&dbname=CJFQ2009.
MLA	刘光明,et al."文蛤特异性过敏原免疫识别的初步研究".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=spkx200901051&dbcode=CJFQ&dbname=CJFQ2009 (2012).