极地产适冷脂肪酶菌株的酶学性质研究

CORC > 集美大学

	极地产适冷脂肪酶菌株的酶学性质研究
	陈兴麟 ; 翁金樑 ; 曾润颖 ; 刘光明
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=twhx201001002&dbcode=CJFQ&dbname=CJFQ2010
	2012-06-05 ; 2012-06-05
关键词	海洋微生物学适冷脂肪酶酶学性质发酵条件极地 marine microbiology cold-adapted lipase characterization of enzyme fermentation condition polar region TQ925
其他题名	Research of enzyme characters of cold-adapted lipase-produced bacteria from the polar
中文摘要	从南北极环境样品中分离到7株产脂肪酶的细菌,经16SrDNA序列分析表明,这些细菌分别属于假交替单胞菌属(Pseudoa lteromonas)和嗜冷杆菌属(Psychrobacter).采用p-NPP法对这7株细菌所产的脂肪酶进行酶学性质研究,结果显示这些酶的最适作用温度在30～40℃、最适作用pH值在7～8之间,在低温下能保持较高的剩余活力,对热敏感,属于适冷脂肪酶.其中假交替单胞菌(Psychrobacter sp.7342)所产脂肪酶具有低温下酶的剩余活力高、有效作用温度和pH范围广、热稳定性较好及对多种金属离子抗性强等特点.该菌株能利用多种单一氮源和碳源产酶,最适产酶温度为25℃.在此基础上进行正交实验分析得到了该菌株的最佳发酵条件为:蛋白胨和淀粉含量各为1.33%,酵母膏含量为0.3%,温度为25℃.; Seven strains of lipase-produced bacteria were isolated and cultured from soil samples of Antarctic and arctic.The results of 16S rDNA sequence analysis showed that 7 bacteria belong to Pseudoalteromonas sp.and Psychrobacter sp.Their enzyme products of bacteria were analysed by p-NPP method.The results showed that optimal temperature of enzyme was 30～40℃,the optimal pH was 7～8.The enzyme maintained a high surplus vitality at low temperature and was heat-sensitive.So the enzyme belonged to low-temperature lipases.The lipase produced by Psychrobacter sp.7342 maintained high enzyme activity at low temperature,had an effective role in a wide range of temperature and pH.It also had better thermal stability and was resisted to a variety of metal ions.The strain can use a variety of single carbon and nitrogen produce enzyme,the optimum temperature for enzyme production is 25℃.Based on the orthogonal experiment,the best fermentation condition of the strain was preliminarily found to be:Tryptone 1.33%,starch 1.33%,yeast 0.3%,at 25℃.; 【作者单位】国家海洋局第三海洋研究所、国家海洋局生物与遗传资源重点实验室；集美大学生物工程学院；【作者英文名】CHEN Xing-lin1,WENG Jin-liang2,ZENG Run-ying1,LIU Guang-ming2(1.Key Laboratory for Marine Biogenetic Resources,SOA,Xiamen 361005,China;2.College of Biological Engineering,Jimei University,Xiamen 361021,China)
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/18190]
专题	集美大学
推荐引用方式 GB/T 7714	陈兴麟,翁金樑,曾润颖,等. 极地产适冷脂肪酶菌株的酶学性质研究[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=twhx201001002&dbcode=CJFQ&dbname=CJFQ2010,2012, 2012.
APA	陈兴麟,翁金樑,曾润颖,&刘光明.(2012).极地产适冷脂肪酶菌株的酶学性质研究.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=twhx201001002&dbcode=CJFQ&dbname=CJFQ2010.
MLA	陈兴麟,et al."极地产适冷脂肪酶菌株的酶学性质研究".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=twhx201001002&dbcode=CJFQ&dbname=CJFQ2010 (2012).