空肠弯曲菌的磁捕获_-荧光PCR检测方法的建立

CORC > 集美大学

	空肠弯曲菌的磁捕获_-荧光PCR检测方法的建立
	刘光明 ; 苏文金 ; 蔡慧农 ; 谢明星 ; 刘棠 ; 彭小莉
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=shwu200502034&dbcode=CJFQ&dbname=CJFQ2005
	2012-06-05 ; 2012-06-05
关键词	空肠弯曲菌免疫磁珠捕获荧光PCR 检测 Campylobacter jejuni immunomagnetic ca pture fluorescent PCR detection R446.5
其他题名	Establishment of Immunomagnetic Capture-Fluorescent PCR Detection Metho d for Campylobacter jejuni
中文摘要	为提高畜禽类食品中空肠弯曲菌的检出率和灵敏度 ,应用抗血清和磁性微珠首次制备弯曲菌免疫磁珠 ,利用弯曲菌免疫磁珠直接捕获检样中目的菌 ,不需要增菌培养 ;通过荧光PCR检测鞭毛蛋白A(flaA)基因和或马尿酸酶 (hipO)基因 ,首次建立空肠弯曲菌的磁捕获_荧光聚合酶链反应 (IMC_FPCR)方法。IMC_FPCR法检测空肠弯曲菌方法简便易行 ,可在 2 4h内完成 ,特异性好 ,检测低限达到 10cfu mL ,抗干扰性强。IMC_FPCR方法可望解决非可培养状态的空肠弯曲菌检测难题 ,是一种适用于检验检疫、卫生防疫和农产品安全检验等领域的快速方法。; In order to develop a rapid method which can check Campylobacter jejuni in animal and poultry foods nicely, an immun omag netic capture-fluorescent PCR (IMC-FPCR) method was established in this paper. T he reported method involves isolation of the target pathogen by immunocapture pr ior to the fluorescent PCR step, therefore the immunomagnetic-beads for Campylob acter were developed, and two groups of primer/probe, which targeted for the spe cies special sequence of fl gene and hipO gene for Campylobacter jejuni were d esigned. The immunomagnetic capture-fluorescent PCR assay amplification of the h ipO gene and fl gene for detection of Campylobacter jejuni was firstly reporte d in this paper. Result indicated that IMC-FPCR method permits direct detection of the pathogen without an enrichment step and can be performed in approximately 24 h. The assay results are positive for all of the isolates of Campylobacter jejuni (3 isolates, including type strain ATCC 33560 and ATCC8341) with a det ecti on limit of approximately 10 cfu/mL, are negative for Campylobacter coli and sev eral other bacteria. IMC-FPCR assay provide not only a rapid, sensitive method f or quantitative detection of Campylobacter jejuni, but also an important met hod for detecting of Campylobacter jejuni of viable but non-culturerable (VN C) state.; 【作者单位】厦门出入境检验检疫局；集美大学生物工程学院；厦门出入境检验检疫局厦门361012汕头大学生物学系；汕头515063；厦门361021；厦门361012；【作者英文名】LIU Guang-Ming 1, 2*, SU Wen-Jin 3, CAI Hui-Nong 3, XIE Ming-Xing 1, LIU Tang 1 and PENG Xiao-Li 1 1. Xiamen Entry-Exit Inspection and Quarantine Bureau, Xiamen 361012, China 2. Department of Biology, Shantou University, Shantou 515063, China3. Schoo
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/18187]
专题	集美大学
推荐引用方式 GB/T 7714	刘光明,苏文金,蔡慧农,等. 空肠弯曲菌的磁捕获_-荧光PCR检测方法的建立[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=shwu200502034&dbcode=CJFQ&dbname=CJFQ2005,2012, 2012.
APA	刘光明,苏文金,蔡慧农,谢明星,刘棠,&彭小莉.(2012).空肠弯曲菌的磁捕获_-荧光PCR检测方法的建立.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=shwu200502034&dbcode=CJFQ&dbname=CJFQ2005.
MLA	刘光明,et al."空肠弯曲菌的磁捕获_-荧光PCR检测方法的建立".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=shwu200502034&dbcode=CJFQ&dbname=CJFQ2005 (2012).