厦门海域产蛋白酶浮游细菌的多样性研究

CORC > 集美大学

	厦门海域产蛋白酶浮游细菌的多样性研究
	刘光明 ; 曾润颖 ; 林则勇 ; 曾胤新
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=gjsx200809019&dbcode=CJFQ&dbname=CJFQ2008
	2012-06-05 ; 2012-06-05
关键词	蛋白酶浮游细菌多样性厦门海域 protease planktonic bacteria diversity Xiamen sea area Q93
其他题名	Study on diversity of protease-producing planktonic bacteria in the Xiamen sea area
中文摘要	从厦门海域分离筛选到33株产蛋白酶浮游细菌,并通过对其16S 核糖体 RNA(16s rRNA)基因进行聚合酶链式反应(PCR)扩增和限制性片段长度多态性(RFLP)分析,初步研究了该海域产蛋白酶浮游细菌的多样性。研究发现,这33株菌可分为12种操作分类单元(OTU),其中 OTU-Ⅰ和 OTU-Ⅱ分别包含8株和12株菌株,分别占总菌数的24.2%和36.4%,为优势分离类群。分别从12种 OTU 中随机选取1株细菌作为代表进行16S rRNA 基因测序。序列分析结果显示,属于 OTU-Ⅶ的细菌为假交替单胞菌属(Pseu-doalteromonas sp.),而包括 OTU-Ⅰ和 OTU-Ⅱ在内的9种 OTU 都属于弧菌属(Vibrio sp.),表明弧菌在厦门海域产蛋白酶浮游细菌中占优势。初步的生理检测显示,这些细菌的适宜生长温度为16%左右,适宜生长盐度范围为3%～5%NaCl。; The diversity of protease-producing planktonic bacteria in the Xiamen sea area was studied through a total of 33 pro- tease-producing planktonic bacterial strains isolated from this sea area based on the method of polymerase chain reaction coupled with restriction fragment length polymorphism(PCR-RFLP)and the 16S ribosomal RNA(16S rRNA)gene se- quencing.The results of PCR-RFLP indicate that these 33 strains can be grouped into 12 different operational taxonomic units(OTU).OTU-Ⅰ and OTU-Ⅱ are the predominant OTUs,which contains 8 and 12 strains,respectively,accounting for 24.2% and 36.4% of the total protease-produeing bacteria.A representative strain of each OTU was selected at ran- dom for 16S rRNA gene sequencing.The results show that the bacterial strains of OTU-VIII belong to the genus Pseu- doalteromonas.Nine OTUs,including OTU-Ⅰ and OTU-Ⅱ,belong to the genus Vibrio,suggesting that Vibrio dominate in protease-produeing planktonic bacteria in the Xiamen sea area.The results of physiology testing display that the op’mum growth temperature is about 16℃ and the optimum growth salinity is about 3%～5% NaCl.; 【作者单位】集美大学生物工程学院；国家海洋局海洋生物遗传资源重点实验室；国家海洋局极地科学重点实验室；【作者英文名】Liu Guangming Zeng Runying~* Lin Zeyong Zeng Yinxin~(*) (College of Biological Engineering,Jimei University,Xiamen 361021)(~The Key Laboratory for Marine Biogenetic Resources of State Oceanic Administration,Xiamen 361005)(~(**)The Key Laboratory fo
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/18172]
专题	集美大学
推荐引用方式 GB/T 7714	刘光明,曾润颖,林则勇,等. 厦门海域产蛋白酶浮游细菌的多样性研究[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=gjsx200809019&dbcode=CJFQ&dbname=CJFQ2008,2012, 2012.
APA	刘光明,曾润颖,林则勇,&曾胤新.(2012).厦门海域产蛋白酶浮游细菌的多样性研究.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=gjsx200809019&dbcode=CJFQ&dbname=CJFQ2008.
MLA	刘光明,et al."厦门海域产蛋白酶浮游细菌的多样性研究".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=gjsx200809019&dbcode=CJFQ&dbname=CJFQ2008 (2012).