利用5’LongSAGE标签分析蜜蜂肌球蛋白调节性轻链基因MLC-2选择性转录起始位点

CORC > 集美大学

	利用5’LongSAGE标签分析蜜蜂肌球蛋白调节性轻链基因MLC-2选择性转录起始位点
	周沫玲 ; 孙亮先 ; 陈培山 ; 谢宪兵 ; 黄周英 ; 倪辉
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jxnd201001031&dbcode=CJFQ&dbname=CJFQ2010
	2012-06-05 ; 2012-06-05
关键词	5’LongSAGE 标签 MLC-2基因西方蜜蜂转录起始位点启动子 5’LongSAGE tag MLC-2 gene Apis mellifera transcription start site(TSS) promoter S891
其他题名	Identification of Alternative Transcription Start Sites of MLC-2 Gene in Apis mellifera by 5’LongSAGE
中文摘要	使用西方蜜蜂雄蜂头部5’LongSAGE文库中的肌球蛋白调节性轻链基因MLC-2的标签序列,在蜜蜂全基因组序列上定位了该基因的转录起始位点(TSS),并进而预测了其启动子的结构。结果显示:蜜蜂MLC-2基因存在17个TSS,其中16个定位于MLC-2基因开放阅读框上游100bp的范围内。MLC-2基因的各TSS的使用效率不同,其中有3个优势TSS,由之起始的转录本分别占该基因总转录本数量的9.76%、54.47%和11.38%。从碱基组成来看,蜜蜂MLC-2基因TSS的第1个碱基为A、G、T、C的概率分别为58.8%、29.4%、0和11.8%。MLC-2基因的启动子结构预测结果表明,在TSS上游的300bp区域内有3个核心启动子元件。研究结果对研究蜜蜂MLC-2基因的表达调控与确定其全长cDNA序列具有重要意义。; The transcription initiation start site(TSS) of a predicted honeybee MLC-2 gene(myosin regulatory light chain 2)was localized on the genome sequence of Apis mellifera with the head of drone’s 5’Long-SAGE tags mapping to genome sequences in this study,and the structure of promoter was predicted.The results showed that there were 17 TSSs that were assigned to MLC-2 gene model and 16 tags that mapped in a 100 bp-region upstream of reference start codon.Interestingly,the authors found that those TSSs displayed different initiation efficiency and the MLC-2 mRNA transcripts were started from 3 dominant TSSs,which initiated 9.76%,54.47% and 11.38% respectively of the total transcripts of MLC-2 gene in adult drone head.When base composition of the first base of the 17 alternative TSSs was considered,the authors found that A,G,T and C accounted for 58.8%,29.4%,0 and 11.8%,respectively.Then,the promoter structure of MLC-2 gene was computational analyzed and 3 core promoter elements were predicted in DNA region of 300 bp upstream of TSSs.The precise identification of TSSs was fundamental for the establishment of full-length cDNA sequence and expression regulation of honeybee MLC-2 gene.; 【作者单位】集美大学生物工程学院；泉州师范学院；【作者英文名】ZHOU Mo-ling1,2,SUN Liang-xian2,CHEN Pei-shan2,XIE Xian-bing2,HUANG Zhou-ying2,NI Hui1(1.College of Bioengineering,Jimei University,Xiamen 361021,China;2.Quanzhou Nornal University,Quanzhou 362000,China)
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/18125]
专题	集美大学
推荐引用方式 GB/T 7714	周沫玲,孙亮先,陈培山,等. 利用5’LongSAGE标签分析蜜蜂肌球蛋白调节性轻链基因MLC-2选择性转录起始位点[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jxnd201001031&dbcode=CJFQ&dbname=CJFQ2010,2012, 2012.
APA	周沫玲,孙亮先,陈培山,谢宪兵,黄周英,&倪辉.(2012).利用5’LongSAGE标签分析蜜蜂肌球蛋白调节性轻链基因MLC-2选择性转录起始位点.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jxnd201001031&dbcode=CJFQ&dbname=CJFQ2010.
MLA	周沫玲,et al."利用5’LongSAGE标签分析蜜蜂肌球蛋白调节性轻链基因MLC-2选择性转录起始位点".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jxnd201001031&dbcode=CJFQ&dbname=CJFQ2010 (2012).