巴戟天中一种多糖的分离与结构表征

CORC > 集美大学

	巴戟天中一种多糖的分离与结构表征
	何传波 ; 李琳 ; 汤凤霞 ; 熊何健
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=gdxh200912015&dbcode=CJFQ&dbname=CJFQ2009
	2012-06-05 ; 2012-06-05
关键词	巴戟天多糖分离结构表征 Morinda officinalis How Polysaccharide Isolation Structure characterization R284.1
其他题名	Isolation and Structure Characterization of Polysaccharide from Morinda officinalis How
中文摘要	以巴戟天的根为原料,经热水浸提、Sevag法除蛋白、乙醇沉淀和DEAE-Sepharose CL-6B离子交换柱层析,得到一种水溶性的巴戟天多糖(MOPI-3).通过UV、IR、NMR、GC-MS、高碘酸氧化、Smith降解和甲基化等物理化学方法对MOPI-3的纯度、理化性质和组成结构进行表征.结果表明,MOPI-3分子量为36061,是一种由阿拉伯糖、半乳糖和葡萄糖组成的杂多糖,以α-1,3-吡喃葡萄糖和α-1,4-吡喃半乳糖为主链,平均每5个葡萄糖连接一个半乳糖,每个重复单元具有一个支链,支链由3个呋喃阿拉伯糖以α-1,3-键型组成,连接在主链葡萄糖的6位碳上,MOPI-3含有乙酰基,连接在主链半乳糖的2位碳原子上.; Morinda officinalis How is a rare Chinese traditional herbal medicine.A water soluble polysaccharide(MOPI-3)was obtained from Morinda officinalis How,by hot water extraction,deproteination with Sevag method,precipitation with ethanol,and fractionation through DEAE-Sepharose CL-6B ion exchange chromatography.Using UV,IR,NMR,GC-MS,periodate oxidation,Smith degradation and methylation reaction,the primary structure of MOPI-3 was identified.The results show that,MOPI-3 is highly purified with average molecular weight of 35309 determined by high performance gel permeation chromatography.And it is a heteropolysaccharide composed of Ara,Gal and Glc.The backbone of MOPI-3 was linked by α-1,3-pyran-glucose and α-1,4-pyran-galactose,and the molar ratio of glucose to galactose was 5∶1.There was one side chain composed by three α-1,3-furan-arabinoses linked onto C6 of glucose on backbone in each repeat unit,and the O-acetyl group was linked onto C2 of galactose.; 【作者单位】集美大学生物工程学院；华南理工大学轻工与食品学院；【作者英文名】HE Chuan-Bo1,LI Lin2,TANG Feng-Xia1,XIONG He-Jian1(1.School of Bioengineering,Jimei University,Xiamen 361021,China;2.School of Light Chemistry and Food Science,South China University of Technology,Guangzhou 510640,China)
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/18113]
专题	集美大学
推荐引用方式 GB/T 7714	何传波,李琳,汤凤霞,等. 巴戟天中一种多糖的分离与结构表征[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=gdxh200912015&dbcode=CJFQ&dbname=CJFQ2009,2012, 2012.
APA	何传波,李琳,汤凤霞,&熊何健.(2012).巴戟天中一种多糖的分离与结构表征.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=gdxh200912015&dbcode=CJFQ&dbname=CJFQ2009.
MLA	何传波,et al."巴戟天中一种多糖的分离与结构表征".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=gdxh200912015&dbcode=CJFQ&dbname=CJFQ2009 (2012).