大黄鱼ubc9基因的克隆和组织表达

CORC > 集美大学

	大黄鱼ubc9基因的克隆和组织表达
	周鹏 ; 张子平 ; 王艺磊 ; 谢芳靖 ; 邹志华
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=swjt200908020&dbcode=CJFQ&dbname=CJFQ2009
	2012-06-05 ; 2012-06-05
关键词	ubc9基因小泛素相关修饰物 RT-PCR 大黄鱼 ubc9 gene SUMO RT-PCR Large yellow croaker Q78
其他题名	Cloning and Characterization of ubc9 Gene of Large Yellow Croaker Larimichthys crocea and Its Expression Pattern
中文摘要	类泛素化(sumoylation)是一种重要的转录后修饰过程。激活的SUMO(small ubiquitin-related modifer)和E2结合酶结合稳定后共价结合到底物上,从而完成对底物的类泛素化。UBC9(ubiquitin-conjugating enzyme)作为惟一的E2结合酶,在完成类泛素化中起着重要作用。从已构建的大黄鱼性腺线性化cDNA文库中筛选出ubc9同源片段,用SMART-RACE方法克隆得到了846bp的全长cDNA序列。该序列编码一个由158个氨基酸组成的蛋白。该蛋白序列与已知的UBC9高度同源,含UBC保守结构域和UBC9激活位点区域。实时定量PCR分析ubc9基因在各组织器官的表达,结果发现,ubc9基因在大黄鱼的性腺中大量表达。推测UBC9在大黄鱼性腺发育中起重要的生物学作用。; Sumoylation is a post-translational modification in which SUMO (small ubiquitin-related modifer) proteins are covalently attached to their substrates. As a sole sumoylation E2 enzyme of SUMO,UBC9 is absolutely required for SUMO conjugation. In this study,a fragment cDNA of ubc9 from previous constructed normalized gonad cDNA library from large yellow croaker Larimichthys crocea was employed,and then cloned its full length cDNA which contains 846 bp and coding a protein of 158 amino acid using SMART-RACE PCR amplification. The deduced protein sequence was highly homologue to UBC9s reported in other species which contains a UBC conserve domain and a UBC9 active motif. Expression pattern analysis using real time PCR revealed that ubc9 mRNA abundantly expressed in gonad tissue of large yellow croaker and implied that UBC9 could play important roles in gonad development.; 【作者单位】集美大学水产学院省高校水产科学技术与食品安全重点实验室；美国得克萨斯州立大学化学与生化系；【作者英文名】Zhou Peng1 Zhang Ziping2 Wang Yilei1 Xie Fangjing1 Zou Zhihua1 (1Key Laboratory of Science and Technology for Aquaculture and Food Safety of Fujian Province University,Fisheries College/Fisheries Biotechnology Institute,Jimei University,Xiamen 361021
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/16442]
专题	集美大学
推荐引用方式 GB/T 7714	周鹏,张子平,王艺磊,等. 大黄鱼ubc9基因的克隆和组织表达[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=swjt200908020&dbcode=CJFQ&dbname=CJFQ2009,2012, 2012.
APA	周鹏,张子平,王艺磊,谢芳靖,&邹志华.(2012).大黄鱼ubc9基因的克隆和组织表达.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=swjt200908020&dbcode=CJFQ&dbname=CJFQ2009.
MLA	周鹏,et al."大黄鱼ubc9基因的克隆和组织表达".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=swjt200908020&dbcode=CJFQ&dbname=CJFQ2009 (2012).