嗜酸乳酸杆菌生物学特性研究及其发酵参数优化

CORC > 集美大学

	嗜酸乳酸杆菌生物学特性研究及其发酵参数优化
	黄沧海
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz200501004&dbcode=CJFQ&dbname=CJFQ2005
	2012-06-05 ; 2012-06-05
关键词	嗜酸乳酸杆菌生物学特性发酵参数 Lactobacillus acidophilus biological characteristics fermentation parameters S816.7
其他题名	Study on Biological Characteristics of Lactobacillus acidophilus CGMCC0842 in Vitro and the Optimization of Its Fermentation Parameters
中文摘要	采用均匀设计法优化嗜酸乳酸杆菌的发酵培养基, 研究一株可作为益生素使用的嗜酸乳酸杆菌CGMCC0842的生物学特性, 包括菌体生长曲线、耐热存活率、耐酸存活率、贮藏存活率等. 结果表明, 嗜酸乳酸杆菌的活菌浓度在开始时缓慢上升, 3h后从105 CFU/mL迅速上升, 第18h超过1011 CFU/mL, 到第32h, 细菌数量则缓慢下降, 菌体的适宜收获期宜在发酵后18~23h. 嗜酸乳酸杆菌的75℃处理15min耐热存活率为62.5%, 贮藏1个月的存活率为59 %, pH2.0处理6h的存活率69.5%. 优化的发酵参数为: 时间, 20h; 葡萄糖, 110g/L; 蔗糖, 10g/L; 胰蛋白胨, 5g/L; 酵母浸粉, 30g/L; 柠檬酸盐, 12g/L. 研究表明该乳酸杆菌CGMCC0842是一株优良的饲用益生素菌种.; Some important characteristics of Lactobacillus acidophilus CGMCC0842 were investigated in vitro to provide data for application in feed additive.The growth curve,heat tolerant survivabillity,acid tolerant surviability,storage survivability and fermentation parameters were studied in this experiment.Fermentation parameters experiment was optimized according to the uniform design,in which there is much less experiment required.The concentration of Lactobacillus acidophilus increased slowly at the beginning.After （3 hours） the concentration grew very quickly from 105 CFU/mL to 1011 CFU/mL. The survivability of Lactobacillus acidophilus of heat resistance（75 ℃,15 min）,acid resistance（pH2.0, 6 h） and storage（1 month）is 62.5 %,69.5%, and 59 % respectively.The optimal fermentation parameter was as follows:culture time, 20 h; glucose, 110 g/L; sucrose,10 g/L;peptone 5 g/L; yeast,30 g/L, and ammonium citrate, 12 g/L. The results showed that this strain of Lactobacillus acidophilus was a good potential strain for feed probiotic preparation.; 【作者单位】集美大学水产学院、水产生物技术研究所福建厦门361021；【作者英文名】HUANG Cang-hai(Fisheries College & Institute of Aquaculture Biotechnology, Jimei University, Xiamen 361021,China)
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/12707]
专题	集美大学
推荐引用方式 GB/T 7714	黄沧海. 嗜酸乳酸杆菌生物学特性研究及其发酵参数优化[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz200501004&dbcode=CJFQ&dbname=CJFQ2005,2012, 2012.
APA	黄沧海.(2012).嗜酸乳酸杆菌生物学特性研究及其发酵参数优化.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz200501004&dbcode=CJFQ&dbname=CJFQ2005.
MLA	黄沧海."嗜酸乳酸杆菌生物学特性研究及其发酵参数优化".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz200501004&dbcode=CJFQ&dbname=CJFQ2005 (2012).