大黄鱼血清IgM纯化及其兔抗血清的制备

CORC > 集美大学

	大黄鱼血清IgM纯化及其兔抗血清的制备
	鄢庆枇 ; 韩一凡 ; 高天翔 ; 庄峙厦 ; 王小如
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=zsck200603022&dbcode=CJFQ&dbname=CJFQ2006
	2012-06-05 ; 2012-06-05
关键词	大黄鱼蛋白A亲和层析法免疫球蛋白兔抗鱼IgM血清酶联免疫吸附分析 Pseudosciaena crocea protein A-sepharose affinity chromatography immunoglobulin M(IgM) rabbit sera anti-IgM ELISA S943
其他题名	Purification of serum IgM from large yellow croaker(Pseudosciaena crocea) and preparation of rabbit sera anti-IgM
中文摘要	分别用饱和硫酸铵二次盐析法和蛋白A亲和层析法对健康大黄鱼(Pseudosciaena crocea)血清中的免疫球蛋白(IgM)进行分离纯化,所得产物用SDS-PAGE进行检测。结果表明,蛋白A亲和层析法可以较好地分离到高纯度的大黄鱼血清IgM,产物的电泳胶中只有重链和轻链2个条带;饱和硫酸铵二次盐析法除了有这2个条带,还有很多杂带,而且蛋白A亲和层析法更为简便、快速,因此用蛋白A亲和层析法分离纯化IgM优于饱和硫酸铵二次盐析法;大黄鱼免疫球蛋白重链的分子量在76 kD左右;轻链分子量在28 kD左右。用纯化的大黄鱼IgM免疫实验兔,获得效价高达1∶40 960的兔抗鱼IgM血清。本实验所建立的蛋白A亲和层析法提取大黄鱼血清IgM可以方便、快捷地获得高纯度的产物,适合在实验室中纯化鱼类IgM。本研究所制备的兔抗大黄鱼IgM血清可为今后的相关研究工作打下基础。; The serum IgM of healthy large yellow croaker（Pseudosciaena crocea） was purified by twice salt out of saturated(NH4)2SO4 and protein A-sepharose affinity chromatography.The products were examined by SDS-PAGE electrophoresis.The results showed that the product of protein A-sepharose affinity chromatography had only two bands（heavy chain and light chain） in SDS-PAGE,while the product of twice salt out of saturated(NH4)2SO4 had several other bands;moreover,using protein A-sepharose affinity chromatography to purify IgM was more simple,convenient and fast,so protein A-sepharose affinity chromatography was better than twice salt out of saturated(NH4)2SO4 in terms of the purification of IgM;the molecular weight of heavy chain and light chain of Pseudosciaena crocea IgM were 76?kD and 28?kD respectively.Sera anti-IgM of Pseudosciaena crocea had been prepared by repeatedly immunized New Zealand rabbits with purified IgM,and the titers of anti-sera obtained up to 1∶40?960 examined by indirect ELISA.The results indicated that protein A-sepharose affinity chromatography was feasible in purification of IgM of Pseudosciaena crocea.The anti-sera obtained could be used in correlative studies in future.; 【作者单位】集美大学水产学院；中国海洋大学水产学院；厦门大学化学化工学院；厦门大学化学化工学院福建厦门361021；福建厦门361021；山东青岛266003；福建厦门361005；【作者英文名】YAN Qing-pi~(1),HAN Yi-fan~(1,2),GAO Tian-xiang~(2),ZHUANG Zhi-xia~(3),WANG Xiao-ru~(3)(1.Fisheries College,Jimei University,Xiamen 361021,China;?2.Fisheries College,Ocean University of China,Qingdao 266003,China;?3.College of Chemistry & Chemical En
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/12512]
专题	集美大学
推荐引用方式 GB/T 7714	鄢庆枇,韩一凡,高天翔,等. 大黄鱼血清IgM纯化及其兔抗血清的制备[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=zsck200603022&dbcode=CJFQ&dbname=CJFQ2006,2012, 2012.
APA	鄢庆枇,韩一凡,高天翔,庄峙厦,&王小如.(2012).大黄鱼血清IgM纯化及其兔抗血清的制备.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=zsck200603022&dbcode=CJFQ&dbname=CJFQ2006.
MLA	鄢庆枇,et al."大黄鱼血清IgM纯化及其兔抗血清的制备".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=zsck200603022&dbcode=CJFQ&dbname=CJFQ2006 (2012).