大黄鱼血清IgM纯化及其兔抗血清的制备 | |
鄢庆枇 ; 韩一凡 ; 高天翔 ; 庄峙厦 ; 王小如 | |
刊名 | http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=zsck200603022&dbcode=CJFQ&dbname=CJFQ2006
![]() |
2012-06-05 ; 2012-06-05 | |
关键词 | 大黄鱼 蛋白A亲和层析法 免疫球蛋白 兔抗鱼IgM血清 酶联免疫吸附分析 Pseudosciaena crocea protein A-sepharose affinity chromatography immunoglobulin M(IgM) rabbit sera anti-IgM ELISA S943 |
其他题名 | Purification of serum IgM from large yellow croaker(Pseudosciaena crocea) and preparation of rabbit sera anti-IgM |
中文摘要 | 分别用饱和硫酸铵二次盐析法和蛋白A亲和层析法对健康大黄鱼(Pseudosciaena crocea)血清中的免疫球蛋白(IgM)进行分离纯化,所得产物用SDS-PAGE进行检测。结果表明,蛋白A亲和层析法可以较好地分离到高纯度的大黄鱼血清IgM,产物的电泳胶中只有重链和轻链2个条带;饱和硫酸铵二次盐析法除了有这2个条带,还有很多杂带,而且蛋白A亲和层析法更为简便、快速,因此用蛋白A亲和层析法分离纯化IgM优于饱和硫酸铵二次盐析法;大黄鱼免疫球蛋白重链的分子量在76 kD左右;轻链分子量在28 kD左右。用纯化的大黄鱼IgM免疫实验兔,获得效价高达1∶40 960的兔抗鱼IgM血清。本实验所建立的蛋白A亲和层析法提取大黄鱼血清IgM可以方便、快捷地获得高纯度的产物,适合在实验室中纯化鱼类IgM。本研究所制备的兔抗大黄鱼IgM血清可为今后的相关研究工作打下基础。; The serum IgM of healthy large yellow croaker(Pseudosciaena crocea) was purified by twice salt out of saturated(NH4)2SO4 and protein A-sepharose affinity chromatography.The products were examined by SDS-PAGE electrophoresis.The results showed that the product of protein A-sepharose affinity chromatography had only two bands(heavy chain and light chain) in SDS-PAGE,while the product of twice salt out of saturated(NH4)2SO4 had several other bands;moreover,using protein A-sepharose affinity chromatography to purify IgM was more simple,convenient and fast,so protein A-sepharose affinity chromatography was better than twice salt out of saturated(NH4)2SO4 in terms of the purification of IgM;the molecular weight of heavy chain and light chain of Pseudosciaena crocea IgM were 76?kD and 28?kD respectively.Sera anti-IgM of Pseudosciaena crocea had been prepared by repeatedly immunized New Zealand rabbits with purified IgM,and the titers of anti-sera obtained up to 1∶40?960 examined by indirect ELISA.The results indicated that protein A-sepharose affinity chromatography was feasible in purification of IgM of Pseudosciaena crocea.The anti-sera obtained could be used in correlative studies in future.; 【作者单位】集美大学水产学院; 中国海洋大学水产学院; 厦门大学化学化工学院; 厦门大学化学化工学院 福建厦门361021; 福建厦门361021; 山东青岛266003; 福建厦门361005;【作者英文名】YAN Qing-pi~(1),HAN Yi-fan~(1,2),GAO Tian-xiang~(2),ZHUANG Zhi-xia~(3),WANG Xiao-ru~(3)(1.Fisheries College,Jimei University,Xiamen 361021,China;?2.Fisheries College,Ocean University of China,Qingdao 266003,China;?3.College of Chemistry & Chemical En |
语种 | 中文 |
内容类型 | 期刊论文 |
源URL | [http://ir.calis.edu.cn/hdl/235041/12512] ![]() |
专题 | 集美大学 |
推荐引用方式 GB/T 7714 | 鄢庆枇,韩一凡,高天翔,等. 大黄鱼血清IgM纯化及其兔抗血清的制备[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=zsck200603022&dbcode=CJFQ&dbname=CJFQ2006,2012, 2012. |
APA | 鄢庆枇,韩一凡,高天翔,庄峙厦,&王小如.(2012).大黄鱼血清IgM纯化及其兔抗血清的制备.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=zsck200603022&dbcode=CJFQ&dbname=CJFQ2006. |
MLA | 鄢庆枇,et al."大黄鱼血清IgM纯化及其兔抗血清的制备".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=zsck200603022&dbcode=CJFQ&dbname=CJFQ2006 (2012). |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论