致病性溶藻弧菌生物膜形成特性研究

CORC > 集美大学

	致病性溶藻弧菌生物膜形成特性研究
	姚刚 ; 覃映雪 ; 邹文政 ; 徐晓津 ; 邢颜丽 ; 纪荣兴 ; 郑天凌 ; 鄢庆枇
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=chan201202005&dbcode=CJFQ&dbname=CJFQTEMP
	2012-06-05 ; 2012-06-05
关键词	溶藻弧菌生物膜环境因子 Vibrio alginolyticus bioflim environment factors S943
其他题名	Characteristics of Vibrio alginolyticus Biofilm Formation
中文摘要	采用改良的微孔板法研究静置培养条件下致病性溶藻弧菌ND-01在酶标板上成膜情况。结果显示,溶藻弧菌的生物膜的OD590在16h达到峰值后,随培养时间延长出现下降;在102～108cfu/mL范围内,不同的初始菌密度对生物膜的OD590影响并不显著;NaCl质量分数为4.5%时,所形成生物膜的OD590最高;30℃,溶藻弧菌的生物膜OD590显著高于其他温度组(P<0.05);在胰蛋白胨大豆肉汤培养液中加入质量分数1.5%的CaCl2,能够显著促进生物膜形成,而加入MgCl2对于生物膜的影响则不显著;经过大黄鱼表皮黏液包被后,96孔板中生物膜的OD590显著高于其他部位组织提取液包被组(P<0.01)。致病性溶藻弧菌ND-01能够在聚苯乙烯材料的96孔酶标板表面形成稳定而明显的生物膜,且其生物膜的形成与多种环境因素有着密切关系。; A modified microtiter-plate test was introduced to determine biofilm formation of pathogenic Vibrio alginolyticus ND-01 on the surface of the polystyrene 96-well microtiter plate.Results showed that OD590 of V.alginolyticus strain ND-01 biofilm was found to peak after 16 h;within the 102~108cfu/mL,bacterial concentrations of V.alginolyticus exhibited no significantly effect on the biofilm formation;that the OD590 values of bacterial biofilm increased with the incubation temperatures of 4~30 ℃ and peaked at 30 ℃(P<0.05);and that the OD590 values of bacterial biofilm fluctuated with different NaCl concentrations and optimized at 1% to 7%,the remarkably higher OD590 values at pH 7~9,and peaked at pH 7.Addition of 1.5% CaCl2 significantly promoted the bacterial biofilm formation while MgCl2 played no significantly role;The OD590 of large yellow croaker the biofilm developed on the surface of the microtiter plate coated by the mucus layer on the skin of large yellow croaker was significant(P<0.01).The results showed that V.alginolyticus strain ND-01 developed stable and evident biofilm,the biofilm formation was remarkably affected by environmental factors,such as temperature,pH,NaCl concentration,and Ca2+level.The results also showed that the bacterial biofilm formation of V.alginolyticus was governable,which would be useful for the epidemic control in fish culture.; 【作者单位】厦门大学生命科学学院；集美大学水产学院；【作者英文名】YAO Gang1,2,QIN Ying-xue2,ZOU Wen-zheng2,XU Xiao-jin2,XING Yan-li2,JI Rong-xing2,ZHENG Tian-ling1,YAN Qing-pi2( 1.Life Science College,Xiamen University,Xiamen 361005,China;2.Fisheries College,Jimei University,Xiamen 361021,China )
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/12356]
专题	集美大学
推荐引用方式 GB/T 7714	姚刚,覃映雪,邹文政,等. 致病性溶藻弧菌生物膜形成特性研究[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=chan201202005&dbcode=CJFQ&dbname=CJFQTEMP,2012, 2012.
APA	姚刚.,覃映雪.,邹文政.,徐晓津.,邢颜丽.,...&鄢庆枇.(2012).致病性溶藻弧菌生物膜形成特性研究.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=chan201202005&dbcode=CJFQ&dbname=CJFQTEMP.
MLA	姚刚,et al."致病性溶藻弧菌生物膜形成特性研究".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=chan201202005&dbcode=CJFQ&dbname=CJFQTEMP (2012).