香蕉种质资源亲缘关系的ISSR分析

CORC > 集美大学

	香蕉种质资源亲缘关系的ISSR分析
	姚锦爱 ; 蔡鸿娇 ; 石妞妞 ; 甘林 ; 杨秀娟 ; 陈福如
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=fjnx201201008&dbcode=CJFQ&dbname=CJFQTEMP
	2012-06-05 ; 2012-06-05
关键词	香蕉亲缘关系 ISSR 聚类分析 Banana(Musa spp) genetic relationship ISSR cluster analysis S668.1
其他题名	Genetic Relationship among Banana(Musa spp.) Germplasms Revealed by ISSR Analysis
中文摘要	利用ISSR标记对3个基因型(AAA,AA和ABB)共35份香蕉种质资源的遗传多样性和亲缘关系进行了分析,筛选出的6条ISSR引物共扩增出38条带,扩增片段长度为200～2 000bp,27条具有DNA多态性,多态性为71.05%,遗传距离为4.30%～33.33%。用UPGMA聚类分析结果显示,以遗传距离25%为阈值,35份香蕉种质可分成6个类群,除贡蕉(AA)和金粉1号(ABB)分别归为Ⅲ和Ⅳ两个类群之外,其余33份种质分为4个类群,其中Ⅰ类群包含着来自不同地方的基因型为AAA和ABB的22份香蕉种质。基因型为AAA和ABB的香蕉种质并不被简单地划分成两类,部分基因型相同的香蕉种质之间没有表现出紧密的亲缘关系。本研究所用的6条引物能把香蕉种质区分开,ISSR分子标记对研究香蕉种质资源的亲缘关系是有效的。; Genetic diversity and relationship among 35 banana germplasms involving 3 genotypes(i.e.,AAA,AA and ABB) were studied by using the Inter-Simple Sequence Repeat(ISSR) method.As a result,6 ISSR primers were selected,38 bands amplified between 200 bp and 2,000 bp,27 bands detected for polymorphic DNA,and the percentage of polymorphic DNA 71.05% of polymorphic DNA were found.The genetic distances among the banana cultivars were between 4.30% and 33.33%.A cluster analysis was performed applying the unweighted pair group method on arithmetic averages.The banana germplasms were grouped into 6 clusters based on the genetic distance of 25%.Except for Gongjiao(AA) and Jinfen1(ABB),which were grouped into Cluster III and IV,respectively,the remaining 33 germplasms were classified into 4 clusters,in which Cluster I contained 22 germplasms from different localities and genetypes(AAA and ABB).All germplasms with genotypes AAA and ABB could not be divided into 2 clusters.There appeared no close genetic relationship among some banana germplasms with same genotypes.The germplasms could be discriminated by the 6 selected primers,and ISSR was considered as an effective method for studying genetic relationship among different banana germplasms resources.; 【作者单位】福建省农业科学院植物保护研究所；集美大学水产学院；【作者英文名】YAO Jin-ai1,CAI Hong-jiao2,SHI Niu-niu1,GAN Lin1,YANG Xiu-juan1,CHEN Fu-ru1(1.Institute of Plant Protection,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China; 2.Fisheries College of Jimei University,Xiamen,Fujian 361021,China)
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/12352]
专题	集美大学
推荐引用方式 GB/T 7714	姚锦爱,蔡鸿娇,石妞妞,等. 香蕉种质资源亲缘关系的ISSR分析[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=fjnx201201008&dbcode=CJFQ&dbname=CJFQTEMP,2012, 2012.
APA	姚锦爱,蔡鸿娇,石妞妞,甘林,杨秀娟,&陈福如.(2012).香蕉种质资源亲缘关系的ISSR分析.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=fjnx201201008&dbcode=CJFQ&dbname=CJFQTEMP.
MLA	姚锦爱,et al."香蕉种质资源亲缘关系的ISSR分析".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=fjnx201201008&dbcode=CJFQ&dbname=CJFQTEMP (2012).