日本对虾抗白斑病子三代的抗白斑综合征病毒感染能力及免疫特性

CORC > 集美大学

	日本对虾抗白斑病子三代的抗白斑综合征病毒感染能力及免疫特性
	纪荣兴 ; 邹文政 ; 鄢庆枇 ; 龚三妹 ; 叶兆弘
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=sckx200801016&dbcode=CJFQ&dbname=CJFQ2008
	2012-06-05 ; 2012-06-05
关键词	日本对虾白斑综合征病毒人工感染免疫指标 Penaeus japonicus white spot syndrome virus artificial infection immune parameter S945.4
其他题名	Immunogenicity and WSSV resistance of anti-WSSV Penaeus japonicus F3
中文摘要	为了更好地进行日本对虾抗病良种的选育,本文用浓度为每毫升1×105个病毒粒子的对虾白斑综合征病毒(white spot syndrome virus,WSSV)粗提液注射感染抗白斑病日本对虾子三代和普通日本对虾,观察其死亡率,同时测定WSSV感染后0,4,24,48和96h血淋巴酚氧化酶(PO)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、超氧化物歧化酶(SOD)活力和蛋白含量。结果显示:注射WSSV14d内抗病子三代和普通对虾的平均死亡率分别为15%和85%,前者的抗病保护率达82.4%;感染前前者的各项免疫指标均高于后者,且PO,AKP和蛋白含量存在极显著差异(P<0.01);感染后两者血淋巴检测的5项指标在96h内的变化趋势基本相似,除ACP以外,抗病子三代的其他指标都比普通日本对虾的高。研究结果表明,所选育的抗白斑病日本对虾子三代能够有效地抵抗对虾白斑综合征病毒的侵染,其免疫指标也高于普通日本对虾。; Disease-resistant thoroughbred breeding is considered to be a feasible means to control white spot syndrome of cultured shrimps.For a better understanding of the immunogenicity and WSSV resistance ability of anti-WSSV Penaeus japonicus F3,so that some constructive information can be obtained to improve the breeding of disease-resistant shrimp,the anti-WSSV P.japonicus F3 and the healthy normal shrimps were injected with white spot syndrome virus(WSSV) suspension with a density of 1×105 virus per mL.The mortality of the injected shrimp was recorded and the hemolymphs of shrimps from both groups were collected at 0,4,24,48,96 h post-injection for the evaluation of the immunoparameters such as PO,ACP,AKP,SOD and protein concentration.The results showed that: the mortalities of anti-WSSV F3 and the control shrimp were 15% and 85% at 14 d post-injection,respectively,and the protective rate of anti-WSSV F3 was increased by 82.4%;before injection,the values of all of the immunoparameters in the hemolymphs of the anti-WSSV P.japonicus F3 were higher than those of the control shrimps,and the values of PO and AKP activities as well as the protein concentration exhibited extremely significant difference(P<0.01) from those of control group;five immunity indices exhibited the similar trends with the values of the anti-WSSV F3 always higher than those of the control shrimps except ACP.The results indicate that anti-WSSV P.japonicus F3 can effectively protect themselves from WSSV by the stronger immune function.The results also indicate the promising future of further disease-resistant thoroughbred breeding of P.japonicus.; 【作者单位】集美大学水产学院福建省高校水产科学技术与食品安全重点实验室；集美大学水产学院福建省高校水产科学技术与食品安全重点实验室福建厦门361021；福建厦门361021；【作者英文名】JI Rong-xing,ZOU Wen-zheng,YAN Qing-pi,GONG San-mei,YE Zhao-hong (Key Laboratory of Science and Technology for Aquaculture and Food Safety,Fisheries College of Jimei University,Xiamen 361021,China)
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/12287]
专题	集美大学
推荐引用方式 GB/T 7714	纪荣兴,邹文政,鄢庆枇,等. 日本对虾抗白斑病子三代的抗白斑综合征病毒感染能力及免疫特性[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=sckx200801016&dbcode=CJFQ&dbname=CJFQ2008,2012, 2012.
APA	纪荣兴,邹文政,鄢庆枇,龚三妹,&叶兆弘.(2012).日本对虾抗白斑病子三代的抗白斑综合征病毒感染能力及免疫特性.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=sckx200801016&dbcode=CJFQ&dbname=CJFQ2008.
MLA	纪荣兴,et al."日本对虾抗白斑病子三代的抗白斑综合征病毒感染能力及免疫特性".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=sckx200801016&dbcode=CJFQ&dbname=CJFQ2008 (2012).