60Co-γ射线辐照坛紫菜叶状体及单克隆培养的研究

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	60Co-γ射线辐照坛紫菜叶状体及单克隆培养的研究
	纪德华 ; 陈昌生 ; 郑伟刚 ; 徐燕 ; 梁宝 ; 麻红英
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=twhx200502007&dbcode=CJFQ&dbname=CJFQ2005
	2012-06-05 ; 2012-06-05
关键词	海洋生物坛紫菜 60Co-γ辐照单克隆培养 marine organism Porphyra haitanensis 60Co-γ irradiation monoclone culture S968.4
其他题名	Study of 60Co-γ irradiation and monoclone culture in thallus of Porphyra haitanensis
中文摘要	本文报道了坛紫菜叶状体经60Coγ射线辐照后,用酶解方法获得离体细胞,观察了体细胞的分裂分化及进行单克隆培养叶状体.结果显示:体细胞发育和分化的趋势分为正常叶状体、畸形叶状体、细胞团、丝状体和根丝细胞5种方式;通过单克隆的培养,初步筛选出1个抗逆性较强、生长速度较快的新品系D,该品系1m以上长度的叶状体平均生长速度为9.4cm/d,快的可达11.5cm/d.对新品系的子一代叶状体再度酶解和观察,获得纯系的丝状体.; This article reports the study of cleavage and differentiation and monoclone culture of the somatic cells which were enzymic-degraded from thallus of Porphyra haitanensis after inducing mutation by （）60Co-γ ray irradiation. It shows that the somatic cells developed into five different types, including normal thallus, macrocephalic thallus, cell group, conchocelis and rhizoplast. After monoclone culture and primary selection, a resistant variety D which grew rapidly was found in the experiment. After the thallus length of variety D reached at 1m, its growth rate is 9.4cm/d, and the fastest growth rate can reach 11.5cm/d. The F 1 generation thallus of the new variety was reenzymic-degraded to obtain purebred conchocelis.; 【作者单位】集美大学水产学院；集美大学水产学院福建厦门361021；福建厦门361021；福建厦门361021；【作者英文名】JI De-hua,CHEN Chang-sheng-ZHENG Wei-gang,XU Yan, LIANG Bao,MA Hong-ying(Fisheries College, Jimei University, Xiamen 361021, China)
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/12278]
专题	集美大学
推荐引用方式 GB/T 7714	纪德华,陈昌生,郑伟刚,等. 60Co-γ射线辐照坛紫菜叶状体及单克隆培养的研究[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=twhx200502007&dbcode=CJFQ&dbname=CJFQ2005,2012, 2012.
APA	纪德华,陈昌生,郑伟刚,徐燕,梁宝,&麻红英.(2012).60Co-γ射线辐照坛紫菜叶状体及单克隆培养的研究.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=twhx200502007&dbcode=CJFQ&dbname=CJFQ2005.
MLA	纪德华,et al."60Co-γ射线辐照坛紫菜叶状体及单克隆培养的研究".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=twhx200502007&dbcode=CJFQ&dbname=CJFQ2005 (2012).