借助mtDNA控制区序列分析金鱼与不同地域鲫的亲缘关系

CORC > 集美大学

	借助mtDNA控制区序列分析金鱼与不同地域鲫的亲缘关系
	朱雪莲 ; 王志勇 ; 韩志强
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=dlsc201002010&dbcode=CJFQ&dbname=CJFQ2010
	2012-06-05 ; 2012-06-05
关键词	金鱼鲫不同地域 mtDNA控制区序列亲缘关系 goldfish Carassius auratus localitiy mitochondrial DNA D-loop region phylogenetic relationship S917.4
其他题名	The phylogenetic relationship between goldfish and crucian carp Carassius auratus in different regions based on mtDNA D-loop region sequence
中文摘要	用PCR法克隆测定了4个品种金鱼(草金、文种、龙种和蛋种)、6个不同水域鲫与银鲫的m tDNA控制区部分序列,并对之进行比较分析。结果表明:测得的金鱼与鲫的序列长度均为471 bp,银鲫为472bp;在所用样品中共检测到10种单倍型,金鱼4品种间序列无差异,共享1种单倍型;11尾鲫(来自6个不同水域)呈现8种单倍型,4尾银鲫(来自2个不同水域)共享1种单倍型。聚类分析结果表明,金鱼隶属于鲫的一支,银鲫呈独立的另一支。对金鱼与不同地域鲫的遗传距离测定结果表明:金鱼与嘉兴南湖的鲫亲缘关系最近(遗传距离D=0.00426),其次为盐城射阳湖鲫(D=0.00534)、淮河鲫(D=0.00641)、鄱阳湖鲫(D=0.00749),再次为黄河下游鲫(D=0.01073),与闽江鲫亲缘关系较远(D=0.01291)。据此推测,金鱼起源于长江流域的鲫。; The phylogenetic relationship between crucian carp Carassius auratus and goldfish was studied by partial mitochondrial DNA D-loop region sequencing.Fragments of 472-471 bp at the 5’ end of mitochondrial DNA D-loop region were sequenced in 11 individuals of the crucian carp from 6 localities and 15 individuals of four major lines of goldfish.The sequence of the goldfish showed high similarity to the crucian carp from Yangtze River drainage basin with the genetic distance from 0.00426 to 0.00749.The minimal value of genetic distance(0.00426) was detected between goldfish and the crucian carp from Jiaxing Nan lake,while relatively high value was detected between goldfish and C.auratus from the Yellow River(0.01073) and from Minjiang River(0.01291).The sequence of Carassius auratus gibelio was found to be clustered into a separate clade,indicating that goldfish may originate from Yangtze River drainage basin.; 【作者单位】集美大学水产学院水产科学技术与食品安全重点实验室；集美大学学报编辑部；浙江海洋学院水产学院；【作者英文名】ZHU Xue-lian1,2,WANG Zhi-yong1,HAN Zhi-qiang3 (1.Key Laboratory of Science and Technology for Aquaculture and Food Safety,Fisheries College,Jimei University,Xiamen 361021,China;2.Editiorial Department of Journal of Jimei University,Xiamen 361021,Chin
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/12216]
专题	集美大学
推荐引用方式 GB/T 7714	朱雪莲,王志勇,韩志强. 借助mtDNA控制区序列分析金鱼与不同地域鲫的亲缘关系[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=dlsc201002010&dbcode=CJFQ&dbname=CJFQ2010,2012, 2012.
APA	朱雪莲,王志勇,&韩志强.(2012).借助mtDNA控制区序列分析金鱼与不同地域鲫的亲缘关系.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=dlsc201002010&dbcode=CJFQ&dbname=CJFQ2010.
MLA	朱雪莲,et al."借助mtDNA控制区序列分析金鱼与不同地域鲫的亲缘关系".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=dlsc201002010&dbcode=CJFQ&dbname=CJFQ2010 (2012).