利用AFLP指纹技术研究中国沿海真鲷群体的遗传变异和趋异

CORC > 集美大学

	利用AFLP指纹技术研究中国沿海真鲷群体的遗传变异和趋异
	王志勇 ; 王艺磊 ; 林利民 ; 洪惠馨 ; 张雅芝 ; 邱淑贞 ; 岡本信明
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=sckx200104000&dbcode=CJFQ&dbname=CJFQ2001
	2012-06-05 ; 2012-06-05
关键词	真鲷群体扩增片段长度多态性指纹遗传变异与趋异 Pagrus major population Amplified Fragments Length Polymorphism fingerprinting(AFLP) genetic variation and divergence. S917
其他题名	Genetic variation and divergence of Pagrus major from China seas using AFLP fingerprinting
中文摘要	用AFLP指纹技术对中国沿海真鲷群体进行遗传变异和趋异分析 ,使用了 6对引物 :E -ACC/M -CAT、E -AGG/M -CTG、E -AGG/M -CTT、E -AAC/M -CTA、E -ACA/M -CTG、E -AAC/M -CAA ,在威海、厦门和北部湾海区三个野生群体共 42个体中检出了 6 46个扩增位点 (片段大小 5 0～ 40 0bp) ,其中 70 %位点在群体内或群体间呈现多态。三个群体的多态位点比例变化在 5 8.4%～ 6 4.0 % ,遗传相似系数变化在 0 .842 5～0 .82 0 0 ,以威海群体的遗传变异量最大 ,北部湾群体的最小。三群体间遗传距离和UPGMA谱系图显示 ,厦门海区与北部湾的真鲷差异较小 ,可以认为是同一个亚种群的不同群体 ,黄海真鲷与前二个群体差异比较大 ,属于另一个不同的亚种群。; AFLP fingerprinting was used to detect genetic variation and divergence of three wild stocks of Red Sea Bream, Pagrus major, from the Yellow Sea (Weihai), the East China Sea (Xiamen) and the South China Sea (Beibuwan). A total of 42 individuals were studied using six primer pairs (E-ACC/M-CAT, E-AGG/M-CTG, E-AGG/M-CTT, E-AAC/M-CTA, E-ACA/M-CTG and E-AAC/M-CAA). 452 (70.0%) of 646 selectively amplified fragments ranging in size from 50 bp to 400 bp were polymorphic. The mean percentages of polymorphic bands of the three geographic stocks in question varied from 58.4% to 64.0%, and the genetic similarities estimated within stocks varied from 0.8425 to 0.8200. The maximum and the minimum polymorphism occurred in the samples from the Yellow Sea population and the South China Sea population, respectively. The genetic distances between stocks and UPGMA dendrogram indicated that the genetic divergence between the East China Sea stocks and the South China Sea stocks of P.major was small, suggesting that they belong to the same subpopulation, and the Yellow Sea stocks was separated from the other two stocks, belonging to a different subpopulation.; 【作者单位】集美大学水产学院!福建厦门361021；东京水产大学资源育成学科!日本东京108-8477；【作者英文名】WANG Zhi-yong1, WANG Yi-lei1, LIN Li-min1, HONG Hui-xin1, ZHANG Ya-zhi1, KHOO Sock Kean2, OKAMOTO Nobuaki2 (1. Fisheries College, Jimei University, Xiamen, Fujian 361021, China; 2. Department of Aquatic Biosciences, Tokyo University of Fisheries, Ko
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/12202]
专题	集美大学
推荐引用方式 GB/T 7714	王志勇,王艺磊,林利民,等. 利用AFLP指纹技术研究中国沿海真鲷群体的遗传变异和趋异[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=sckx200104000&dbcode=CJFQ&dbname=CJFQ2001,2012, 2012.
APA	王志勇.,王艺磊.,林利民.,洪惠馨.,张雅芝.,...&岡本信明.(2012).利用AFLP指纹技术研究中国沿海真鲷群体的遗传变异和趋异.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=sckx200104000&dbcode=CJFQ&dbname=CJFQ2001.
MLA	王志勇,et al."利用AFLP指纹技术研究中国沿海真鲷群体的遗传变异和趋异".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=sckx200104000&dbcode=CJFQ&dbname=CJFQ2001 (2012).