以pH敏感相分离高分子为载体的酶联荧光免疫分析法测定人IgG

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	以pH敏感相分离高分子为载体的酶联荧光免疫分析法测定人IgG
	林鹏 ; 郑洪 ; 杨黄浩 ; 杨薇 ; 张长弓 ; 许金钩
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=gdxh200307015&dbcode=CJFQ&dbname=CJFQ2003
	2012-06-05 ; 2012-06-05
关键词	pH敏感相分离高分子酶联荧光免疫分析人IgG pH-Sensitive phase separating polymer Enzyme-linked fluorescence immunoassay Human IgG O657.3
其他题名	Enzyme-linked Fluorescence Immunoassay for Human IgG by Using a pH-Sensitive Phase Separating Polymer as Carrier
中文摘要	Poly(N-isopropylacrylamide-N,N-dimethyl aminopropyl methacrylamide) [P(NIP-DMAPM)], a novel linear water-soluble pH-sensitive polymer whose phase transition pH is about 7.4 at 37 ℃, was synthesized. P(NIP-DMAPM) polymer precipitated out of water above a critical pH 7.4 at 37 ℃ and redissolved at pH below 7.4. P(NIP-DMAPM) was used as a novel separation carrier for the reactants in immunoassay and a enzyme-linked fluorescence immunoassay for human IgG was built. The linear range of determination was 0~1 700 ng/mL and the limit of detection was 2.2 ng/mL. The sensitivity of this method was close to that of traditional ELISA by using same reactants. However, the assay was much faster. This method has been applied to the determination of the human IgG levels in human serum with satisfactory results.; 【作者单位】厦门大学化学系；分析科学教育部重点实验室；厦门大学抗癌研究中心；分析科学教育部重点实验室厦门361005；集美大学水产学院；厦门361021；厦门361005；厦门361005；【作者英文名】LIN Peng 1,2, ZHENG Hong 1, YANG Huang-Hao 1, YANG Wei 1, ZHANG Chang-Gong 3, XU Jin-Gou 1* (1. Key Laboratory of Analytical Science of MOE, Department of Chemistry, Xiamen University, Xiamen 361005, China; 2. Aquaculture College, Jimei University, X
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/12177]
专题	集美大学
推荐引用方式 GB/T 7714	林鹏,郑洪,杨黄浩,等. 以pH敏感相分离高分子为载体的酶联荧光免疫分析法测定人IgG[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=gdxh200307015&dbcode=CJFQ&dbname=CJFQ2003,2012, 2012.
APA	林鹏,郑洪,杨黄浩,杨薇,张长弓,&许金钩.(2012).以pH敏感相分离高分子为载体的酶联荧光免疫分析法测定人IgG.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=gdxh200307015&dbcode=CJFQ&dbname=CJFQ2003.
MLA	林鹏,et al."以pH敏感相分离高分子为载体的酶联荧光免疫分析法测定人IgG".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=gdxh200307015&dbcode=CJFQ&dbname=CJFQ2003 (2012).