金鱼三倍体诱导技术的初步研究

CORC > 集美大学

	金鱼三倍体诱导技术的初步研究
	宋振荣 ; 王良宏 ; 林琪 ; 黄坤森
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz200404004&dbcode=CJFQ&dbname=CJFQ2004
	2012-06-05 ; 2012-06-05
关键词	金鱼三倍体冷休克热休克静水压休克 gold fish triploid cold shock heat shock hydraulic pressure shock S961.2
其他题名	A Prelimilnary Study on Triploid Induction of Gold Fish
中文摘要	运用冷休克、热休克、静水压三种方法抑制金鱼受精卵第二次减数分裂,诱导金鱼三倍体.结果表明:金鱼三倍体诱导率明显受压力、处理温度、处理起始时间以及处理持续时间等因子的影响;在22℃水温下,受精8min后,在0℃冷休克条件下,持续处理14min的三倍体诱导率可达25.5%;受精10min后,在39℃热休克条件下,持续处理1min,三倍体诱导率可达100%;受精4min后,在550kg cm2静水压休克条件下,持续处理3min三倍体诱导率可达28.6%.还对提高金鱼三倍体诱导率和孵化率的方法进行了探讨.; Cold shock,heat shock and hydraulic pressure were used to restrain the second meiosis of the fertilized gold fish eggs and to induce gold fish triploid.It turned out that the gold fish triploid induction rate was obviously affected by the pressure,the treatment temperature,the beginning and continuing treatment time and so on.The triploid induction rate could reach 25 % 8 minutes after fertilization with 0 ℃ cold shock when the continuing treatment time being 14 minutes in 22 ℃ water,and 10 minutes after fertilization with (39 ℃) heat shock when the continuing treatment time being 1 minute ,it could get to 100 %.It could also be 28.6 % 4 minutes after fertilization,with 550 kg/cm~2hydraulic pressure shock when the continuing treatment time being 3 minutes.How to increase the rate of the gold fish triploid induction and hatch was also discussed.; 【作者单位】集美大学水产学院水产生物技术研究所；闽南渔村；福建省水产研究所；集美大学水产学院水产生物技术研究所福建厦门361021；福建漳州363000；福建厦门361012；福建厦门361021；【作者英文名】SONG Zhen-rong~1,WANG Liang-hong~2,LIN Qi~3,HUANG Kun-shen~1(1.Fisheries College & Institute of Aquaculture Biotechnology,Jimei University,Xiamen 361021,China; 2.Minnan Fishing Village,Zhangzhou 363000,China;3.Fisheries Research Institute of Fujian
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/11993]
专题	集美大学
推荐引用方式 GB/T 7714	宋振荣,王良宏,林琪,等. 金鱼三倍体诱导技术的初步研究[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz200404004&dbcode=CJFQ&dbname=CJFQ2004,2012, 2012.
APA	宋振荣,王良宏,林琪,&黄坤森.(2012).金鱼三倍体诱导技术的初步研究.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz200404004&dbcode=CJFQ&dbname=CJFQ2004.
MLA	宋振荣,et al."金鱼三倍体诱导技术的初步研究".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz200404004&dbcode=CJFQ&dbname=CJFQ2004 (2012).