杂色鲍血清凝集素的初步研究

CORC > 集美大学

	杂色鲍血清凝集素的初步研究
	冯建军 ; 王艺磊 ; 关瑞章
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz200404001&dbcode=CJFQ&dbname=CJFQ2004
	2012-06-05 ; 2012-06-05
关键词	杂色鲍凝集素理化性质 Haliotis diversicolor lectin physical and chemical characteristics S917
其他题名	A Study on the Lectin Isolated from the Serum of Haliotis diversicolor supertexta
中文摘要	研究结果表明,杂色鲍血清中自然存在凝集素,该凝集素能够识别和凝集多种不同的细菌和人及动物的红细胞,其中对人体O型和B型血细胞的凝集效价显著高于A型和AB型血细胞(P<0.05);Ca2+能促进其凝集活性,而Mg2+则抑制其凝集活性;凝集作用不同程度地受反应介质的pH、离子强度及反应时间等因素的影响;该凝集素不被透析,在95℃、pH2.0～10.0时仍保持凝集活性,抗RNase、DNase、SDS和乙醚,对蛋白酶K、糖氧化剂NaIO4敏感;凝集素活性能被D-树胶醛糖、L-鼠李糖特异性抑制,D-果糖、D-木糖对其也有显著的抑制作用.; A natural lectin detected from the serum of Haliotis diversicolor supertexta had agglutinating activity for various bacteria and erythrocytes of animal and human.Compared with type A and type AB human erythrocytes,type O and type B human erythrocytes had a significant increase in agglutination titer. The lectin could be ativated by the concentration of ion,reactin time and pH value of the buffer.The lectin could not be dialyzed.The agglutinating activity kept stability under the conditions of 95 ℃,pH 2～10.The lectin was sensitive to sugar oxidant NaIO4 and protease K,but not sensitive to RNase,DNase,SDS and lipid solvent aether.L-Rhamnose,D-Fructose,and D-Xylose had a significant inhibition on the agglutinating activity,especially the former two.; 【作者单位】集美大学水产学院水产生物技术研究所；集美大学水产学院水产生物技术研究所福建厦门361021中国科学院研究生院水生生物研究所湖北武汉430072；福建厦门361021；福建厦门361021中国科学院研究生院水生生物研究所湖北武汉430072；【作者英文名】FENG Jian-jun~(1,2), WANG Yi-lei~1,GUAN Rui-zhang~(1,2)(1.Fisheries College & Institute of Aquaculture Biotechnology, Jimei University, Xiamen 361021,China; 2.Graduate School of the Chinese Academy of Sciences,Institute of Hydrobiology,Wuhan 4300
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/11880]
专题	集美大学
推荐引用方式 GB/T 7714	冯建军,王艺磊,关瑞章. 杂色鲍血清凝集素的初步研究[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz200404001&dbcode=CJFQ&dbname=CJFQ2004,2012, 2012.
APA	冯建军,王艺磊,&关瑞章.(2012).杂色鲍血清凝集素的初步研究.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz200404001&dbcode=CJFQ&dbname=CJFQ2004.
MLA	冯建军,et al."杂色鲍血清凝集素的初步研究".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz200404001&dbcode=CJFQ&dbname=CJFQ2004 (2012).