养殖鲟鱼软骨中硫酸软骨素的优化提取工艺研究

CORC > 集美大学

	养殖鲟鱼软骨中硫酸软骨素的优化提取工艺研究
	徐传屯 ; 关瑞章 ; 郑江 ; 黄世玉 ; 黄文树
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=shyw200903006&dbcode=CJFQ&dbname=CJFQ2009
	2012-06-05 ; 2012-06-05
关键词	鲟鱼软骨硫酸软骨素提取工艺 sturgeon cartilage chondroitin sulfate extraction technology TQ464
其他题名	Studies on the optimized extration technique of the chondroitin sulfate from cartilage of cultured sturgeon
中文摘要	目的以养殖鲟鱼软骨为原料,提取硫酸软骨素、筛选提取工艺条件。方法用稀碱-盐解-酶解法提取硫酸软骨素,并对各工艺中影响提取效果的不同因素进行正交实验。结果碱提取最佳工艺组合为:碱浓度5%,料液比1∶6,碱提取时间3h;盐解最佳工艺组合为:盐浓度4%,盐解温度90℃,盐解时间20min;酶解最佳工艺组合为:酶浓度0.4%,酶解温度52℃,酶解时间2.5h。结论采用上述组合提取硫酸软骨素的得率为31.56%,显著高于(P<0.05)传统工艺的25.86%。; Purpose The chondoitin sulfate was isolated from cartilage of cultured Chinese sturgeon through a method of dilute alkali-enzyme hydrolysis.The extraction technique of chondroitin sulfate was improved.MethodsThe Chondoitin sulfate was isolated through a method of dilute alkali-enzyme hydrolysis.ResultsTo extract the chondroitin sulfate from sturgeon cartilage in dilate alkali,the best combination of extracting technique was:the 5% alkali concentration,the 1∶6 weight proportion,and the time of 3 hours.To extract the chondroitin sulfate from sturgeon cartilage in dilute salt,the best combination was the 4% salt concentration,the temperature of 90,and the time of 20 minutes.In enzymeolysis,the best combination was 0.4% enzyme concentration,the temperature of 52 ℃,and the time of 2.5 hours.ConclusionThe yield of chondroitin sulfate increased significantly(P<0.05)after the optimization of the technique.; 【作者单位】集美大学水产学院；【作者英文名】XU Chuan-tun,GUAN Rui-zhang,ZHENG Jiang,HUANG Shi-yu,HUANG Wen-shu(Fisheries School,Jimei University,Xiamen 361021,China)
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/11873]
专题	集美大学
推荐引用方式 GB/T 7714	徐传屯,关瑞章,郑江,等. 养殖鲟鱼软骨中硫酸软骨素的优化提取工艺研究[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=shyw200903006&dbcode=CJFQ&dbname=CJFQ2009,2012, 2012.
APA	徐传屯,关瑞章,郑江,黄世玉,&黄文树.(2012).养殖鲟鱼软骨中硫酸软骨素的优化提取工艺研究.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=shyw200903006&dbcode=CJFQ&dbname=CJFQ2009.
MLA	徐传屯,et al."养殖鲟鱼软骨中硫酸软骨素的优化提取工艺研究".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=shyw200903006&dbcode=CJFQ&dbname=CJFQ2009 (2012).