硫酸软骨素测定方法的研究进展

CORC > 集美大学

	硫酸软骨素测定方法的研究进展
	徐传屯 ; 关瑞章 ; 郑江
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz200801011&dbcode=CJFQ&dbname=CJFQ2008
	2012-06-05 ; 2012-06-05
关键词	硫酸软骨素软骨素二糖氨基己糖葡萄糖醛酸硫酸基含量测定 chondroitin sulfate chondroitn disaccharid glucosamine glucuronic acid sulfate ion content determination Q53-3
其他题名	Progress Research of the Determination Methods of Chondroitin Sulfate
中文摘要	硫酸软骨素的测定方法按其测定指标主要分为软骨素二糖、氨基己糖、葡萄糖醛酸、硫酸基和阴性基团5类.比较这5种指标认为:软骨素二糖是硫酸软骨素的组成单位,与硫酸软骨素含量的相关性最高;氨基己糖和葡萄糖醛酸是组成软骨素二糖的单糖,与硫酸软骨素含量的相关性较高;硫酸基受软骨素二糖硫酸化程度影响大,与硫酸软骨素含量相关性低;阳离子染料对硫酸软骨素分子上阴性基团的选择专一性不强,阴性基团与硫酸软骨素含量的相关性很低.从准确度看,以软骨素二糖为测定指标的高效液相色谱法准确度最高,适于质量控制和微量分析;分别以氨基己糖和葡萄糖醛酸为测定基础的E lson-Morgan法和咔唑法准确度相对较高,适于一般分析;而以硫酸基和阴性基团为基础的方法准确度相对偏低.; The determination methods of chondroitin sulfate mainly include chondroitn disaccharid,glucosamine,glucuronic acid,sulfate ion,and negative ionogen according to the determination indicators of these methods.Chondroitn disaccharide is the composition of chondroitin sulfate,and it has the highest pertinence to chondroitin sulfate.Glucosamine and glucuronic acid are monosaccharides that compose chondroitn disaccharide,and they have a higher pertinence to chondroitin sulfate.The content of Sulfate ion is badly influenced by the sulfation of chondroitin sulfate,and its pertinence to chondroitin sulfate is lower.The negative ion also has a lower pertinence to chondroitin sulfate for the positive ion dye can’t linkage characteristic with chondroitin sulfate.As far as accuracy is concerned,HPLC which determines chondroitn disaccharide has the highest accuracy,and it can apply to quality control and microanalysis;Elson-Morgan method and carbazole method have a higher accuracy,and they can apply to generic analysis;other methods have a lower accuracy.; 【作者单位】集美大学水产学院；集美大学水产学院福建厦门361021；福建省高校水产科学技术与食品安全重点实验室；福建厦门361021；集美大学水产生物技术研究所；【作者英文名】XU Chuan-tun1,2,3,GUAN Rui-zhang1,2,3,ZHENG Jiang1,2,3(1.Fisheries College,Jimei University,Xiamen 361021,China;2.Key Laboratory of Science and Technology forAquaculture and Food Safety(Jimei University),Fujian Province,Xiamen 361021,China;3.Institut
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/11865]
专题	集美大学
推荐引用方式 GB/T 7714	徐传屯,关瑞章,郑江. 硫酸软骨素测定方法的研究进展[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz200801011&dbcode=CJFQ&dbname=CJFQ2008,2012, 2012.
APA	徐传屯,关瑞章,&郑江.(2012).硫酸软骨素测定方法的研究进展.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz200801011&dbcode=CJFQ&dbname=CJFQ2008.
MLA	徐传屯,et al."硫酸软骨素测定方法的研究进展".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz200801011&dbcode=CJFQ&dbname=CJFQ2008 (2012).