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	F-box蛋白COI1稳定性调控机制
	陈娟 ; 姚瑞枫 ; 陈泓宇 ; 李海鸥 ; 谢道昕 ; 闫建斌 ; CHEN Juan ; YAO RuiFeng ; CHEN HongYu ; LI HaiOu ; XIE DaoXin ; YAN JianBin
	2016-03-30 ; 2016-03-30
关键词	茉莉素 COI1蛋白 SCF复合体 蛋白稳定性 蛋白酶体 zinc finger proteins one-hybrid system protein-DNA interactions Q946
其他题名	Regulation Mechanism of Protein Stability of the F-box Protein COI1 from Tomato and Tobacco
中文摘要	拟南芥F-box蛋白COI1(Coronatine insensitive 1)与ASK1(Arabidopsis serine/Threonine kinase 1)蛋白及CUL1(CULLIN1)蛋白等结合形成SCFCOI1泛素连接酶复合体.COI1感知茉莉素信号、进而调控植物一系列的防御反应和生长发育过程.虽然多种作物的COI1同源蛋白已经被相继鉴定,但是其自身蛋白水平的调控机制仍然未知.本文重点研究了蔬菜作物番茄(Solanum lycopersicum)和经济作物烟草(Nicotiana attenuata)中COI1蛋白稳定性的调控机制.结果证明,这两种作物的COI1蛋白通过与ASK1的相互作用而得到稳定,表明形成SCFCOI1复合体可能有助于COI1蛋白的稳定.同时,26S蛋白酶体抑制剂能够明显抑制COI1的降解,说明泛素-蛋白酶体途径参与了其降解过程.这些结果证明在这两个不同物种中,两条互相拮抗的途径共同发挥作用,平衡并稳定COI1蛋白在细胞内的恰当丰度.该研究为深入研究不同作物的茉莉素信号转导调控机制奠定了良好的基础.; Arabidopsis COI1(coronatine insensitive 1), an F-box protein that physically interacts with ASK1(Arabidopsis serine/Threonine kinase 1) protein to form SCFCOI1-E3 ligase complex, perceives jasmonate signals and modulates diverse aspects of the jasmonate-regulated plant defense and developmental processes. The COI1 homologs from various plant species have recently been identified, however, little is known about the molecular mechanism regulating protein stability of these COI1 homologs. In this study, we demonstrated that the COI1 homologs from vegetable crop Solanum lycopersicum and commercial plant Nicotiana attenuata remain stable via interacting with ASK1 protein, suggesting that assembly of SCFCOI1 complexes contributes to the stabilization of COI1 homologs. Meanwhile, the 26 S proteasome inhibitor represses the degradation of COI1 homologs, demonstrating that ubiquitin-proteasome pathway plays a role in degradation of the COI1 homologs. These results suggest that two antagonistic pathways work together to maintain COI1 protein in tomato and tobacco at a suitable abundance for proper biological function.
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://ir.lib.tsinghua.edu.cn/ir/item.do?handle=123456789/148479]
专题	清华大学
推荐引用方式 GB/T 7714	陈娟,姚瑞枫,陈泓宇,等. F-box蛋白COI1稳定性调控机制[J],2016, 2016.
APA	陈娟.,姚瑞枫.,陈泓宇.,李海鸥.,谢道昕.,...&YAN JianBin.(2016).F-box蛋白COI1稳定性调控机制..
MLA	陈娟,et al."F-box蛋白COI1稳定性调控机制".(2016).

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