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	Yin Yang 1(YY1) synergizes with Smad7 to inhibit TGF-β signaling in the nucleus
	YAN XiaoHua ; PAN Jun ; XIONG WanWan ; CHENG MinZhang ; SUN YingYuan ; ZHANG SuPing ; CHEN YeGuang ; YAN XiaoHua ; PAN Jun ; XIONG WanWan ; CHENG MinZhang ; SUN YingYuan ; ZHANG SuPing ; CHEN YeGuang
	2016-03-30 ; 2016-03-30
关键词	TGF-β Smad7 YY1 HDAC1 signaling regulation transcriptional co-repressor Q26
其他题名	Yin Yang 1(YY1) synergizes with Smad7 to inhibit TGF-β signaling in the nucleus
中文摘要	As a prototype of the TGF-βsuperfamily cytokines,TGF-βis well known for its diverse roles in embryogenesis and adult tissue homeostasis.TGF-βevokes cellular responses by signaling mainly through cell membrane receptors and transcription factor R-Smads and Co-Smad(Smad4),while an inhibitory Smad,Smad7,acts as a critical negative regulator of TGF-βsignaling.Smad7 antagonizes TGF-βsignaling by regulating the stability or activity of the receptors or blocking the DNA binding of the functional R-Smad-Smad4 complex in the nucleus.However,the function of Smad7 in the nucleus is not fully understood.Yin Yang 1(YY1)is a ubiquitously expressed transcription factor with multiple functions.It has been reported that YY1 can inhibit Smad-dependent transcriptional responses and TGF-β/BMP-induced cell differentiation independently of its DNA binding ability.In this study,we found that Smad7 interacts with YY1 and the interaction is attenuated by TGF-βsignaling.Reporter assays and target gene expression analyses revealed that Smad7 and YY1 act in concert to inhibit TGF-β-induced transcription in the nucleus.Furthermore,Smad7 could enhance the interaction of YY1 with the histone deacetylase HDAC1.Consistently,YY1 and HDAC1 augmented the transcription repression activity of Smad7 in Gal4-luciferase reporter analysis.Therefore,our findings define a novel mechanism of Smad7 and YY1 to antagonize TGF-βsignaling.; As a prototype of the TGF-β superfamily cytokines, TGF-β is well known for its diverse roles in embryogenesis and adult tis- sue homeostasis. TGF-β evokes cellular responses by signaling mainly through cell membrane receptors and transcription fac- tor R-Smads and Co-Smad(Smad4), while an inhibitory Smad, Smad7, acts as a critical negative regulator of TGF-β signaling. Smad7 antagonizes TGF-β signaling by regulating the stability or activity of the receptors or blocking the DNA binding of the functional R-Smad-Smad4 complex in the nucleus. However, the function of Smad7 in the nucleus is not fully understood. Yin Yang 1(YY1) is a ubiquitously expressed transcription factor with multiple functions. It has been reported that YY1 can inhib- it Smad-dependent transcriptional responses and TGF-β/BMP-induced cell differentiation independently of its DNA binding ability. In this study, we found that Smad7 interacts with YY1 and the interaction is attenuated by TGF-β signaling. Reporter assays and target gene expression analyses revealed that Smad7 and YY1 act in concert to inhibit TGF-β-induced transcription in the nucleus. Furthermore, Smad7 could enhance the interaction of YY1 with the histone deacetylase HDAC1. Consistently, YY1 and HDAC1 augmented the transcription repression activity of Smad7 in Gal4-luciferase reporter analysis. Therefore, our findings define a novel mechanism of Smad7 and YY1 to antagonize TGF-β signaling.
语种	英语 ; 英语
内容类型	期刊论文
源URL	[http://ir.lib.tsinghua.edu.cn/ir/item.do?handle=123456789/148415]
专题	清华大学
推荐引用方式 GB/T 7714	YAN XiaoHua,PAN Jun,XIONG WanWan,et al. Yin Yang 1(YY1) synergizes with Smad7 to inhibit TGF-β signaling in the nucleus[J],2016, 2016.
APA	YAN XiaoHua.,PAN Jun.,XIONG WanWan.,CHENG MinZhang.,SUN YingYuan.,...&CHEN YeGuang.(2016).Yin Yang 1(YY1) synergizes with Smad7 to inhibit TGF-β signaling in the nucleus..
MLA	YAN XiaoHua,et al."Yin Yang 1(YY1) synergizes with Smad7 to inhibit TGF-β signaling in the nucleus".(2016).

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