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	鼻咽癌microRNAs差异表达谱的鉴定
	聂国辉 ; 刘锋 ; 林宜林 ; 陈静 ; 余振东 ; 李晓庆 ; 段鸿芳 ; 路瑞静 ; 赵春娟 ; 胡洪义 ; 梅雪霜 ; NIE Guohui ; LIU Feng ; LIN Yilin ; CHEN Jing ; YU Zhendong ; LI Xiaoqing ; DUAN Hongfang ; LU Ruijing ; ZHAO Chunjuan ; HU Hongyi ; MEI Xueshuang
	2016-03-30 ; 2016-03-30
关键词	鼻咽癌 microRNAs 表达谱 nasopharyngeal carcinoma microRNAs profiles R739.63
其他题名	Idenfication of microRNAs profiles in nasopharyngeal carcinoma
中文摘要	目的:筛选及验证鼻咽癌中microRNAs(miRs)的差异表达谱。方法:通过芯片高通量表达谱分析及大规模微阵列技术,筛选鼻咽癌与炎症组织中差异表达的miRs,并应用RT-QPCR方法验证筛选结果的可信性。结果:鼻咽癌组织与对照组织的miRs存在差异表达,其中差异倍数大于2倍的人属miRs共有144种。RT-QPCR发现与炎症组织相比,癌组织中miRs-34b、miRs-449b、miRs-7-1表达显著下调,而miRs-125b、miRs-184、miRs-196b、miRs-205及miRs-24-1表达上调,结果与芯片分析结果相一致。结论:差异表达的miRs可能与鼻咽癌的发生、发展密切相关,对鼻咽癌miRs表达谱的研究可能为鼻咽癌的早期诊断及治疗提供有力的靶向依据。; Objective:To filtrate and prove the different microRNAs(miRs)profiles in nasopharyngeal carcinoma.Method:Screening the different expressions of miRs between nasopharyngeal carcinoma and the inflammatory tissues by the application of expression profiling of chip high-throughput and large-scale microarray analysis.Then we used RTQPCR technology to prove the accuracy of screening results.Result:There were significant expression differences of miRs between nasopharyngeal carcinoma and the control tissues,144human miRs had 2or more fold the difference ratio.Compared with the inflammatory tissues,we have found that miRs-34b,miRs-449band miRs-7-1significantly low expressed in nasopharyngeal carcinoma,yet miRs-125b,miRs-184,miRs-196b,miRs-205and miRs-24-1expressed high.The results were consistent with the microarray analysis.Conclusion:The difference expressed miRs might be closely related to the process of nasopharyngeal carcinoma,and the research on miRs profiles maybe provide a powerful target basis for early diagnosis and therapy of nasopharyngeal carcinoma.
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://ir.lib.tsinghua.edu.cn/ir/item.do?handle=123456789/148267]
专题	清华大学
推荐引用方式 GB/T 7714	聂国辉,刘锋,林宜林,等. 鼻咽癌microRNAs差异表达谱的鉴定[J],2016, 2016.
APA	聂国辉.,刘锋.,林宜林.,陈静.,余振东.,...&MEI Xueshuang.(2016).鼻咽癌microRNAs差异表达谱的鉴定..
MLA	聂国辉,et al."鼻咽癌microRNAs差异表达谱的鉴定".(2016).

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