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	人组蛋白α-氨基乙酰基转移酶Nat11表达纯化、晶体生长及底物结合研究
	黄嘉欣 ; 李海涛 ; Huang Jiaxin ; Li Haitao
	2016-03-30 ; 2016-03-30
关键词	α-氨基乙酰基转移酶11 重组蛋白表达 蛋白聚集 酶-底物结合 晶体生长 3D transmon Rabi oscillation Ramsey fringe spin echo Q55
其他题名	Expression,Crystallization and Substrate Binding Studies of Human Histone N-terminal Acetyltransferase Nat11
中文摘要	α-氨基乙酰基转移酶11(Nat11)催化组蛋白H4和H2A氨基端乙酰化修饰,发挥着重要的表观遗传调控功能。将人Nat11基因构建到原核表达载体p SUMO中,转化入大肠杆菌BL21(DE3)进行重组表达。通过镍柱亲和层析等一系列体外纯化步骤,获得高纯度Nat11。利用等温滴定量热法(ITC),测得Nat11与底物多肽微摩尔量级结合常数。利用质谱技术,发现纯化后的Nat11结合有大肠杆菌内源产生的乙酰辅酶A或辅酶A,在ITC滴定过程中可以产生对多肽底物的乙酰化修饰,表明纯化获得的Nat11在溶液中具有酶活力。随后,对Nat11进行晶体生长研究,通过初筛优化获得蛋白截短体及底物-酶融合蛋白单晶。; The alpha-amino groups of histones H4 and H2 A can be acetylated by histone N-terminal acetyltransferase 11(Nat11), which plays an important role in epigenetic regulation. The c DNA of human Nat11 was amplified and cloned into p SUMO vector. The resultant construct was transformed into E.coli strain BL21(DE3)for reco mbinant protein expression. Homogenous Nat11 was highly purified through a series of purification procedures including nickel column affinity chromatography. Using isothermal titration calorimetry(ITC), we measured micromolar binding constants between Nat11 and histone H4 peptides. MALDI-TOF mass spectrometry analysis revealed that purified Nat11 was pre-bound with acetyl coenzyme A or coenzyme A that was co-purified from E.coli. After ITC titration using unmodified peptide as ligand, N-acetylated product was detected by mass spectrometry, suggesting that the purified Nat11 is active. We performed crystallization screening and successfully obtained single crystal of a truncate form of Nat11 and substrate-enzyme recombinant protein after optimization.
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://ir.lib.tsinghua.edu.cn/ir/item.do?handle=123456789/147469]
专题	清华大学
推荐引用方式 GB/T 7714	黄嘉欣,李海涛,Huang Jiaxin,等. 人组蛋白α-氨基乙酰基转移酶Nat11表达纯化、晶体生长及底物结合研究[J],2016, 2016.
APA	黄嘉欣,李海涛,Huang Jiaxin,&Li Haitao.(2016).人组蛋白α-氨基乙酰基转移酶Nat11表达纯化、晶体生长及底物结合研究..
MLA	黄嘉欣,et al."人组蛋白α-氨基乙酰基转移酶Nat11表达纯化、晶体生长及底物结合研究".(2016).

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