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	miR-155特异性siRNA增强阿糖胞苷诱导的Burkitt淋巴瘤Raji细胞凋亡
	刘平平 ; 朱锦灿 ; 郑力 ; 刘善淘 ; 谭广销 ; 何冬梅 ; 刘革修 ; LIU Ping-ping ; ZHU Jin-can ; ZHENG Li ; LIU Shan-tao ; TAN Guang-xiao ; HE Dong-mei ; LIU Ge-xiu
	2016-03-30 ; 2016-03-30
关键词	阿糖胞苷 miR-155 小干扰RNA Burkitt淋巴瘤 细胞凋亡 Deficiency of vitamin B12 Cerebellum disease Infection R733.1
其他题名	miR-155-specific siRNA enhances chemosensitivity of Burkitt lymphoma Raji cells to cytosine arabinoside by inducing apoptosis
中文摘要	目的:研究miR-155特异性siRNA增强阿糖胞苷(Ara-C)诱导的Burkitt淋巴瘤Raji细胞凋亡及其机制。方法:Ara-C与miR-155 siRNA单独或者联合干预Raji细胞增殖。qRT-PCR检测脂质体转染siRNA后miR-155的表达;CCK-8法检测细胞增殖;流式细胞术检测细胞凋亡;Western blotting法检测caspase-3的表达。结果:qRT-PCR显示,转染miR-155 siRNA后,Raji细胞的miR-155表达明显下降(P<0.05),Ara-C或miR-155 siRNA单独处理细胞后均显示出增殖抑制作用,而两者联合使用的增殖抑制作用更显著(P<0.05)。各组细胞进行药物干预48 h后行流式细胞术,结果显示Ara-C或miR-155 siRNA单独处理细胞凋亡率分别为(16.5±0.3)%和(14.6±0.3)%,与对照组[(3.6±0.4)%]比较差别有统计学意义(P<0.05),Ara-C+miR-155 siRNA组的凋亡率[(38.4±1.4)%]分别与Ara-C和miR-155 siRNA单独处理组比较,差别有统计学意义(P<0.05)。Ara-C+miR-155siRNA组与Ara-C组及miR-155 siRNA组相比,caspase-3的水平明显增高。结论:miR-155特异性siRNA具有增强阿糖胞苷对Raji细胞增殖抑制及诱导Raji细胞凋亡的作用,其机制与caspase-3凋亡途径有关。; AIM: To investigate the effect of miR-155-specific siRNA alone or in combination with cytosine arabinoside( Ara-C) on the growth and apoptosis of Burkitt lymphoma Raji cells. METHODS: miR-155-specific siRNA and /or Ara-C were used to treat the cells. Quantitative real-time polymerase chain reaction was used to detect the expression of miR-155. The growth of the cells was analyzed by CKK-8 assay. The cell apoptosis was determined by flow cytometry. RESULTS: The miR-155 expression level of the cells transfected with miR-155 siRNA was significantly lower than that in the 2 control groups. Ara-C or miR-155 siRNA alone inhibited the growth of Raji cells in a dose-depend manner.miR-155 siRNA combined with Ara-C produced more inhibition of cell proliferation( P < 0. 05). After treatment for 48 h,the apoptotic rate of Raji cells in miR-155 siRNA + Ara-C group [( 38. 4 �1. 4) %] was higher than that in Ara-C group[( 16.5 �.3) %]and miR-155 siRNA group [( 14.6 �.3) %],with statistically significant difference( P <0.05).The expression of caspase-3 in Ara-C + miR-155 siRNA group was increased significantly as compared with Ara-C group and miR-155 siRNA group. CONCLUSION: miR-155-specific siRNA enhances the chemosensitivity of Raji cells to AraC by inducing apoptosis through the caspase-3 pathway.
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://ir.lib.tsinghua.edu.cn/ir/item.do?handle=123456789/147303]
专题	清华大学
推荐引用方式 GB/T 7714	刘平平,朱锦灿,郑力,等. miR-155特异性siRNA增强阿糖胞苷诱导的Burkitt淋巴瘤Raji细胞凋亡[J],2016, 2016.
APA	刘平平.,朱锦灿.,郑力.,刘善淘.,谭广销.,...&LIU Ge-xiu.(2016).miR-155特异性siRNA增强阿糖胞苷诱导的Burkitt淋巴瘤Raji细胞凋亡..
MLA	刘平平,et al."miR-155特异性siRNA增强阿糖胞苷诱导的Burkitt淋巴瘤Raji细胞凋亡".(2016).

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