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	Lipid profiling of mammalian cells with in situ matrix-assisted laser desorption ionization-mass spectrometry
	ZHANG YanDong ; LI HaiFang ; MA Yuan ; LIN JinMing ; ZHANG YanDong ; LI HaiFang ; MA Yuan ; LIN JinMing
	2016-03-30 ; 2016-03-30
关键词	lipid profiling MALDI-MS linear discriminative analysis Q2-3
其他题名	Lipid profiling of mammalian cells with in situ matrix-assisted laser desorption ionization-mass spectrometry
中文摘要	The analysis of cellular lipids and phospholipids has been of continuously increasing research interest due to the importance of these molecules in psychological process.In this work,a mass spectrometry-based method for direct,in situ analysis of lipids in cells was reported.Mammalian cells were directly cultured on ITO-coated glass and then analyzed by matrix-assisted laser desorption ionization-mass spectrometry(MALDI-MS).The matrix application process was achieved by electrospray coating,which produced a homogenous layer of matrix crystal on the sample.The detection results and reproducibility are satisfactory.With this method,a profile of abundant membrane lipids is generated,which is characteristic of cell type.In conclusion,this in situ MALDI-MS cellular lipid analysis method provides a platform for sensitive and robust molecular profiling of mammalian cells.; The analysis of cellular lipids and phospholipids has been of continuously increasing research interest due to the importance of these molecules in psychological process. In this work, a mass spectrometry-based method for direct, in situ analysis of lipids in cells was reported. Mammalian cells were directly cultured on ITO-coated glass and then analyzed by matrix-assisted laser desorption ionization-mass spectrometry(MALDI-MS). The matrix application process was achieved by electrospray coating, which produced a homogenous layer of matrix crystal on the sample. The detection results and reproducibility are satisfactory. With this method, a profile of abundant membrane lipids is generated, which is characteristic of cell type. In conclusion, this in situ MALDI-MS cellular lipid analysis method provides a platform for sensitive and robust molecular profiling of mammalian cells.
语种	英语 ; 英语
内容类型	期刊论文
源URL	[http://ir.lib.tsinghua.edu.cn/ir/item.do?handle=123456789/145936]
专题	清华大学
推荐引用方式 GB/T 7714	ZHANG YanDong,LI HaiFang,MA Yuan,et al. Lipid profiling of mammalian cells with in situ matrix-assisted laser desorption ionization-mass spectrometry[J],2016, 2016.
APA	ZHANG YanDong.,LI HaiFang.,MA Yuan.,LIN JinMing.,ZHANG YanDong.,...&LIN JinMing.(2016).Lipid profiling of mammalian cells with in situ matrix-assisted laser desorption ionization-mass spectrometry..
MLA	ZHANG YanDong,et al."Lipid profiling of mammalian cells with in situ matrix-assisted laser desorption ionization-mass spectrometry".(2016).

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