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	硫酸-紫外法与苯酚-硫酸法测定千两茶中总多糖的比较
	辛敏 ; 刘轩 ; 詹欣 ; 黄昀 ; 唐劲天 ; 盛军 ; XIN Min ; LIU Xuan ; ZHAN Xin ; HUANG Yun ; TANG Jin-tian ; SHENG Jun
	2016-03-30 ; 2016-03-30
关键词	茶多糖 千两茶 苯酚-硫酸法 硫酸-紫外法,含量测定 tea polysaccharides qianliang tea phenol-sulfuric acid sulfuric acid-UV determination R284.1
其他题名	Determination of Total Polysaccharides in Qianliang Tea by Sulfuric Acid-UV Method and Phenol-Sulfuric Acid Method
中文摘要	目的:比较苯酚-硫酸法与硫酸-紫外法在测定千两茶多糖含量上的差异。方法:千两茶粉经水提醇沉,Sevage法除蛋白后,采用苯酚-硫酸法和硫酸-紫外法测定茶多糖含量。结果:苯酚-硫酸法标准曲线方程为Y=4.755 0X-0.029 5(R2=0.990 4),硫酸-紫外法标准曲线方程为Y=4.770 0X-0.065 1(R2=0.993 1),均在0.02~0.1 g·L-1有良好的线性关系。所测茶多糖质量分数分别为2.00%,3.21%,其差异有统计学意义。结论:硫酸-紫外法作为一种多糖检测新手段,可简便测定多糖含量。; Objective: To compare differences between phenol-sulfuric acid method and sulfuric acid-UV method to determine polysaccharides content in Qianliang tea. Method: The two methods were employed to determine content of polysaccharides in Qianliang tea after water extracting-alcohol precipitating and sevage assays. Result: The total sugar stand curve equation were Y = 4. 755 0X-0. 029 5( R2= 0. 990 4)( by sulfuric acid-UV method) and Y = 4. 770 0X-0. 065 1( R2= 0. 993 1)( by phenol-sulfuric acid method),which showed a good linear relationship range from 0. 02 to 0. 1 g稬-1. The determination of phenol-sulfuric acid method and sulfuric acid-UV method were 2. 00% and 3. 21%,which was statistically significance. Conclusion: As a new method,sulfuric acid-UV method could be a stable and simple method to measure the polysaccharide.
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://ir.lib.tsinghua.edu.cn/ir/item.do?handle=123456789/143097]
专题	清华大学
推荐引用方式 GB/T 7714	辛敏,刘轩,詹欣,等. 硫酸-紫外法与苯酚-硫酸法测定千两茶中总多糖的比较[J],2016, 2016.
APA	辛敏.,刘轩.,詹欣.,黄昀.,唐劲天.,...&SHENG Jun.(2016).硫酸-紫外法与苯酚-硫酸法测定千两茶中总多糖的比较..
MLA	辛敏,et al."硫酸-紫外法与苯酚-硫酸法测定千两茶中总多糖的比较".(2016).

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