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	Production of two monomer structures containing medium-chain-length polyhydroxyalkanoates by beta-oxidation-impaired mutant of Pseudomonas putida KT2442
	Ma, Long ; Zhang, Haifeng ; Liu, Qian ; Chen, Jiong ; Zhang, Jing ; Chen, Guo-Qiang
	2010-10-12 ; 2010-10-12 ; OCT
关键词	PUB Pseudomonas putida Polyhydroxyalkanoates beta-Oxidation 3-Hydroxydodecanoate FADA KNOCKOUT MUTANT ESCHERICHIA-COLI Agricultural Engineering Biotechnology & Applied Microbiology Energy & Fuels
中文摘要	Pseudomonas putida KT2442 produces medium-chain-length (MCL) polyhydroxyalkanoates (PHA) from fatty acids. When gene encoding 3-hydroxyacyl-CoA dehydrogenase which catalyzes long-chain-3-hydroxyacyl-CoA to 3-ketoacyl-CoA, was partially or completely deleted in P. putida KTOY08, the PHA accumulated was shown to contain only two different monomer structures dominated by a monomer of the same chain length as that of the fatty acids fed and another monomer two carbon atoms shorter. Among the PHA copolymers, P(44% 3HD-co-3HDD) containing 44% 3HD and 56% 3HDD was demonstrated to have a melting temperature T-m, all apparent heat of fusion Delta H-m and a Young's modulus E of 75 degrees C, 51 J g(-1) and 2.0 MPa, respectively, the highest among all the MCL PHA Studied. (C) 2009 Elsevier Ltd. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCI LTD ; OXFORD ; THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/81892]
专题	清华大学
推荐引用方式 GB/T 7714	Ma, Long,Zhang, Haifeng,Liu, Qian,et al. Production of two monomer structures containing medium-chain-length polyhydroxyalkanoates by beta-oxidation-impaired mutant of Pseudomonas putida KT2442[J],2010, 2010, OCT.
APA	Ma, Long,Zhang, Haifeng,Liu, Qian,Chen, Jiong,Zhang, Jing,&Chen, Guo-Qiang.(2010).Production of two monomer structures containing medium-chain-length polyhydroxyalkanoates by beta-oxidation-impaired mutant of Pseudomonas putida KT2442..
MLA	Ma, Long,et al."Production of two monomer structures containing medium-chain-length polyhydroxyalkanoates by beta-oxidation-impaired mutant of Pseudomonas putida KT2442".(2010).

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