| The cytoplasmic domain of influenza M2 protein interacts with caveolin-1 | |
| Peng Zou ; Fan Wu ; Lu Lu ; Huang, Jing-He ; Chen, Ying-Hua | |
| 2010-10-12 ; 2010-10-12 | |
| 关键词 | Influenza M2 protein Cytoplasmic domain Multimerization Caveolin-1 IMMUNODEFICIENCY-VIRUS TYPE-1 CHOLESTEROL-BINDING PROTEIN INTEGRAL MEMBRANE-PROTEIN SCAFFOLDING DOMAIN ION-CHANNEL HIV-1 GP41 TAIL EPITOPE MICRODOMAINS IDENTIFICATION Biochemistry & Molecular Biology Biophysics |
| 中文摘要 | The cytoplasmic domain of influenza M2 protein (M2c) consists of 54 amino acid (aa) residues from aa44 to aa97. In this paper, M2c and its deletion mutant M2c(Delta 47-55) were expressed using prokaryotic expression system. First, glutaraldehyde crosslinking assay showed that M2c had multimerization potential mediated by aa47-55. Then, M2c, instead of M2c(Delta 47-55), directed eGFP from the whole cell localization to a predominately perinuclear region in CHO cells, which indicated that aa47-55 of M2c mediated the localization. Moreover, M2c colocalized with caveolin-1 (Cav) when CHO cells were cotransfected with Cav. A caveolin-1 binding motif Phi xxxx Phi xx Phi (Phi represents aromatic amino acid residues) in aa47-55 of M2c was found by sequence alignment and analysis. Further overlay ELISA result showed that M2c, but not M2c(Delta 47-55), bound to prokaryotically expressed cholesterol-free Cav(2-101), which illustrated the interaction could be cholesterol-independent. That was the first report of cellular protein bound to M2c. (C) 2009 Elsevier Inc. All rights reserved. |
| 语种 | 英语 ; 英语 |
| 出版者 | ELSEVIER SCIENCE INC ; NEW YORK ; 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/81860]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | Peng Zou,Fan Wu,Lu Lu,et al. The cytoplasmic domain of influenza M2 protein interacts with caveolin-1[J],2010, 2010. |
| APA | Peng Zou,Fan Wu,Lu Lu,Huang, Jing-He,&Chen, Ying-Hua.(2010).The cytoplasmic domain of influenza M2 protein interacts with caveolin-1.. |
| MLA | Peng Zou,et al."The cytoplasmic domain of influenza M2 protein interacts with caveolin-1".(2010). |
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