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	Development and evaluation of a recombinant CP23 antigen-based ELISA for serodiagnosis of Cryptosporidium parvum
	Wang, Chengmin ; He, Hongxuan ; Duan, Mingxing
	2010-10-12 ; 2010-10-12
关键词	Recombinant CP23 protein Cryptosporidium parvum Indirect ELISA INFECTIOUS-BRONCHITIS VIRUS IMMUNOGLOBULIN-G ANTIBODIES MONOCLONAL-ANTIBODIES IMMUNE-RESPONSES IMMUNOBLOT ANALYSIS ENZYME-IMMUNOASSAY MICE SERUM CALVES SPOROZOITES Parasitology
中文摘要	The CP23 gene of Cryptosporidium parvum strain isolated from Changchun in China was expressed in Escherichia coli BL21 strain and was purified as a recombinant protein. An indirect ELISA assay (CP23-ELISA) for antibody detection was established using the purified recombinant CP23 protein. Antigen coating conditions and serum dilution for the CP23-ELISA were optimized. The S/P ratio of the absorbency value was calculated in the CP23-ELISA to evaluate the serum antibody level of the field cow samples. It indicated that the CP23-ELISA assay, which was more convenient and easier to prepare than traditional methods, was a good candidate for evaluation of C. parvum exposure to domestic animal in field. Crown Copyright (C) 2008 Published by Elsevier Inc. All rights reserved.
语种	英语 ; 英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE ; SAN DIEGO ; 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/81852]
专题	清华大学
推荐引用方式 GB/T 7714	Wang, Chengmin,He, Hongxuan,Duan, Mingxing. Development and evaluation of a recombinant CP23 antigen-based ELISA for serodiagnosis of Cryptosporidium parvum[J],2010, 2010.
APA	Wang, Chengmin,He, Hongxuan,&Duan, Mingxing.(2010).Development and evaluation of a recombinant CP23 antigen-based ELISA for serodiagnosis of Cryptosporidium parvum..
MLA	Wang, Chengmin,et al."Development and evaluation of a recombinant CP23 antigen-based ELISA for serodiagnosis of Cryptosporidium parvum".(2010).

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